IRX1 antibody (AA 95-480)

Catalog No. ABIN7877224

The Rabbit Polyclonal anti-IRX1 antibody (ABIN7877224) specifically detects IRX1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for IRX1 antibody (AA 95-480) (ABIN7877224)

Target: IRX1 (Iroquois Homeobox Protein 1 (IRX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IRX1 Antibody

Binding Specificity: AA 95-480

Purpose: IRX1 Antibody / Iroquois-class homeodomain protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human IRX1 recombinant protein (Position: Q95-A480) was used as the immunogen for the IRX1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IRX1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the IRX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for IRX1

Target: IRX1 (Iroquois Homeobox Protein 1 (IRX1))

Alternative Name: IRX1

Background: IRX1 antibody detects Iroquois-class homeodomain protein IRX1, encoded by the IRX1 gene on chromosome 5p15.33. IRX1 is a transcription factor belonging to the Iroquois homeobox gene family, which regulates embryonic patterning, neuronal differentiation, and organogenesis. IRX1 contains a conserved homeodomain that binds DNA and directs gene expression programs critical for tissue development. In vertebrates, IRX1 contributes to forebrain, heart, limb, and craniofacial patterning. By modulating downstream effectors, it integrates positional cues during embryogenesis, ensuring correct morphogenesis.

IRX1 expression persists in select adult tissues, suggesting roles beyond development, including neuronal plasticity and epithelial regulation. Dysregulation of IRX1 has been linked to oncogenesis. Tumor suppressor roles are observed in gastric cancer, where IRX1 downregulation correlates with poor prognosis, while in other cancers it may function as an oncogene depending on cellular context. Epigenetic silencing of IRX1 through promoter methylation has been reported, highlighting transcriptional repression as a mechanism in cancer progression. IRX1 antibody therefore provides researchers with a means to examine both developmental biology and disease-associated misregulation.

At the molecular level, IRX1 acts as a transcriptional regulator of Wnt, BMP, and Notch signaling pathways, impacting proliferation, differentiation, and apoptosis. In cardiac development, IRX1 contributes to ventricular chamber specification and conduction system maturation. In neural development, it regulates differentiation of specific neuronal subtypes. Animal models with IRX1 knockouts display craniofacial abnormalities, cardiac defects, and disrupted hindbrain segmentation, highlighting its multifaceted role.

Experimentally, IRX1 antibody has applications in immunohistochemistry, immunofluorescence, and western blotting. It is used to track IRX1 expression during embryogenesis, to validate promoter methylation effects in tumors, and to study transcription factor localization in nuclei. NSJ Bioreagents provides IRX1 antibody to facilitate investigations into morphogenetic regulation, cancer epigenetics, and gene regulatory networks.

UniProt: P78414

Pathways: Tube Formation