XPO1 antibody (AA 988-1054)

Catalog No. ABIN7877288

This Rabbit Polyclonal antibody specifically detects XPO1 in WB. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for XPO1 antibody (AA 988-1054) (ABIN7877288)

Target: XPO1 (Exportin 1 (XPO1))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XPO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-XPO1 Antibody

Binding Specificity: AA 988-1054

Purpose: Zebrafish Xpo1 Antibody / Xpo1a / Xpo1b / Exportin 1

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Xpo1 recombinant protein (amino acids H988-L1054) was used as the immunogen for the Zebrafish Xpo1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Xpo1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Xpo1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for XPO1

Target: XPO1 (Exportin 1 (XPO1))

Alternative Name: Xpo1

Background: Zebrafish Xpo1, also known as Exportin 1, is a critical protein involved in the nuclear export of cargo molecules, including RNA and ribosomal proteins. Xpo1 functions as a transport receptor that mediates the export of proteins and RNA from the nucleus to the cytoplasm by recognizing cargo molecules and facilitating their passage through the nuclear pore complex. This process is essential for regulating gene expression, RNA processing, and cellular signaling, as it ensures that molecules required for various cellular processes are efficiently transported to the appropriate cellular compartments.

Zebrafish Xpo1 is an ortholog of the human XPO1 protein, and the two share significant sequence and functional conservation. Similar to human Xpo1, zebrafish Xpo1 is involved in the transport of a wide variety of cargo molecules, including ribosomal subunits and RNA species such as mRNA, tRNA, and snRNA. This makes Xpo1 essential for cellular homeostasis and the proper functioning of many cellular systems, including gene expression, protein synthesis, and stress responses.

Zebrafish Xpo1 exists in multiple isoforms, which may have specialized roles in different tissues or developmental stages. The primary isoform is responsible for most of the transport activity, while other isoforms may be involved in specific cargo recognition or subcellular localization. The diversity of Xpo1 isoforms allows for fine-tuned regulation of nuclear export and contributes to the adaptability of the cellular transport system in zebrafish.

The use of a Zebrafish Xpo1 antibody allows researchers to study the expression, localization, and function of Xpo1 during various stages of zebrafish development. Zebrafish Xpo1 antibody reagents are essential tools for applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA, enabling the detection and analysis of Xpo1 isoforms and their involvement in nuclear export pathways. A Zebrafish Xpo1 antibody can help identify how perturbations in Xpo1 function affect cellular processes like mRNA transport, protein synthesis, and cell signaling.

By using a high-quality Zebrafish Xpo1 antibody, scientists can gain insights into the regulation of nuclear-cytoplasmic transport, a crucial aspect of cellular function that is conserved across species.

UniProt: E7F0E8

Pathways: M Phase