14-3-3 eta antibody (C-Term)

Catalog No. ABIN7877305

The Rabbit Polyclonal anti-14-3-3 eta antibody (ABIN7877305) specifically detects 14-3-3 eta in WB, IF, IHC, ICC and FACS. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for 14-3-3 eta antibody (C-Term) (ABIN7877305)

Target: 14-3-3 eta (YWHAH)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 eta antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-14-3-3 eta Antibody

Binding Specificity: C-Term

Purpose: YWHAH Antibody / 14-3-3 eta

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human YWHAH was used as the immunogen for the YWHAH antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the YWHAH antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the YWHAH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for 14-3-3 eta

Target: 14-3-3 eta (YWHAH)

Alternative Name: YWHAH

Background: YWHAH antibody detects 14-3-3 protein eta, encoded by the YWHAH gene on chromosome 22q12.3. YWHAH antibody is widely applied in studies of signaling, apoptosis, and neurological disorders. YWHAH belongs to the 14-3-3 family of conserved regulatory proteins that bind phosphoserine-containing motifs on target proteins. Through these interactions, YWHAH regulates a wide range of cellular processes including cell cycle progression, apoptosis, signal transduction, and cytoskeletal organization.

Structurally, YWHAH is a ~28 kDa acidic protein that forms homo- or heterodimers with other 14-3-3 family members. Its structure consists of nine alpha-helices that create an amphipathic groove, enabling binding of phosphorylated motifs. These interactions influence localization, stability, and activity of client proteins. YWHAH is ubiquitously expressed, but highest in brain, reflecting its importance in neuronal signaling.

Functionally, YWHAH participates in multiple signaling cascades, including MAPK, PI3K-Akt, and apoptotic pathways. By binding pro-apoptotic proteins like BAD, YWHAH sequesters them in the cytoplasm to promote cell survival. It also modulates cytoskeletal dynamics by interacting with actin- and microtubule-regulatory proteins. YWHAH is involved in dopamine signaling by regulating proteins downstream of dopamine receptors. Researchers use YWHAH antibody to study signal transduction, apoptosis, and neurodegeneration.

Clinically, YWHAH is linked to neurological and psychiatric disorders. Genetic variants have been associated with schizophrenia, bipolar disorder, and Parkinson's disease. Its dysregulation is observed in neurodegenerative diseases such as Alzheimer's, where altered phosphorylation-dependent binding disrupts signaling. YWHAH is also studied in cancer, where its interactions influence cell proliferation and resistance to apoptosis. NSJ Bioreagents supplies YWHAH antibody to support signaling, neuroscience, and oncology research.

Experimentally, YWHAH antibody is used in western blotting to detect the ~28 kDa protein, in immunohistochemistry to analyze brain expression, and in immunofluorescence microscopy to visualize cytoplasmic localization. Co-immunoprecipitation with YWHAH antibody identifies client proteins across multiple pathways.

UniProt: Q04917

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, C21-Steroid Hormone Metabolic Process, Myometrial Relaxation and Contraction