ARL2 antibody (C-Term)

Catalog No. ABIN7877310

The Rabbit Polyclonal anti-ARL2 antibody is suitable to detect ARL2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, FACS, IF and ICC.

Quick Overview for ARL2 antibody (C-Term) (ABIN7877310)

Target: ARL2 (ADP-Ribosylation Factor-Like 2 (ARL2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ARL2 Antibody

Binding Specificity: C-Term

Purpose: ARL2 Antibody / ADP-ribosylation factor-like protein 2

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARL2 was used as the immunogen for the ARL2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ARL2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ARL2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ARL2

Target: ARL2 (ADP-Ribosylation Factor-Like 2 (ARL2))

Alternative Name: ARL2

Background: ARL2 antibody detects ADP-ribosylation factor-like protein 2, a small GTP-binding protein involved in microtubule dynamics, mitochondrial function, and intracellular transport. Encoded by the ARL2 gene on chromosome 11q13.2, this evolutionarily conserved member of the ARF family of GTPases cycles between active GTP-bound and inactive GDP-bound states to regulate cytoskeletal organization and vesicle trafficking. ARL2 participates in essential cellular processes ranging from ciliary assembly to energy metabolism.

Structurally, ARL2 is a 184-amino-acid cytosolic protein of approximately 21 kilodaltons containing the characteristic GTP-binding motifs (G1-G5 boxes) required for nucleotide exchange and hydrolysis. It interacts with cofactors such as cofactor D (TBCD) and cofactor E (TBCE) to modulate tubulin folding and polymerization. ARL2 localizes to both cytoplasm and mitochondria, where it forms complexes that support microtubule stability and mitochondrial fusion.

The ARL2 antibody is widely used in cell biology, neurobiology, and mitochondrial research to study small GTPase signaling, cytoskeletal organization, and intracellular trafficking. Western blot analysis detects a 21 kilodalton band corresponding to ARL2, while immunofluorescence demonstrates cytoplasmic and mitochondrial staining patterns. This antibody provides a dependable tool for monitoring ARL2-mediated control of tubulin dynamics and organelle function.

Functionally, ARL2 regulates the folding of alpha and beta tubulin heterodimers, supporting microtubule assembly critical for intracellular transport and cell division. Within mitochondria, ARL2 influences fusion, morphology, and ATP production by interacting with proteins such as ANT1 and ELMOD2. Dysregulation of ARL2 expression affects mitochondrial integrity, axonal transport, and cell viability. In neurons, ARL2 contributes to microtubule-based trafficking of synaptic vesicles and organelles. The ARL2 antibody enables analysis of these processes and provides insights into cytoskeletal and metabolic coordination. NSJ Bioreagents validates this antibody for its applications, ensuring reliable and sensitive detection for GTPase and mitochondrial studies.

UniProt: P36404

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location