ARL3 antibody (C-Term)

Catalog No. ABIN7877311

The Rabbit Polyclonal anti-ARL3 antibody (ABIN7877311) specifically detects ARL3 in WB, IF, FACS and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ARL3 antibody (C-Term) (ABIN7877311)

Target: ARL3 (ADP-Ribosylation Factor-Like 3 (ARL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-ARL3 Antibody

Binding Specificity: C-Term

Purpose: ARL3 Antibody / ADP-ribosylation factor-like protein 3

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARL3 was used as the immunogen for the ARL3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ARL3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ARL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ARL3

Target: ARL3 (ADP-Ribosylation Factor-Like 3 (ARL3))

Alternative Name: ARL3

Background: The ARL3 antibody targets ADP-ribosylation factor-like protein 3, a small GTP-binding protein encoded by the ARL3 gene. ADP-ribosylation factor-like protein 3 is a member of the Arf family of GTPases that regulates trafficking of lipid-modified proteins to primary cilia and photoreceptor outer segments. Through its role in ciliary cargo transport, ARL3 ensures the correct localization of proteins essential for sensory signaling and phototransduction. The ARL3 antibody provides a reliable reagent for studying ciliary trafficking, GTPase signaling, and inherited retinal disorders.

ADP-ribosylation factor-like protein 3 cycles between GTP- and GDP-bound states, regulating the release of lipidated cargo from carrier proteins such as PDE6D and UNC119. The ARL3 antibody supports studies that monitor these molecular interactions and visualize ARL3 localization within cilia, photoreceptor cells, and centrosomes. Its activation is mediated by the guanine nucleotide exchange factor ARL13B, while GTP hydrolysis is stimulated by the GTPase-activating protein RP2. Together, these interactions ensure precise spatiotemporal control of cargo delivery.

Mutations in the ARL3 gene cause Joubert syndrome and cone-rod dystrophy, disorders associated with ciliary dysfunction and photoreceptor degeneration. The ARL3 antibody is critical for studying these ciliopathies, enabling detection of expression changes and subcellular mislocalization of mutant proteins. Disruption of ARL3-mediated trafficking leads to accumulation of mislocalized proteins and progressive loss of photoreceptor function.

Beyond the retina, ADP-ribosylation factor-like protein 3 regulates microtubule organization and vesicle transport in other ciliated and non-ciliated cells. The ARL3 antibody supports investigations into these broader cellular functions, including roles in signal transduction and organelle positioning. ARL3 also contributes to lipid modification cycles that control the targeting of myristoylated and prenylated proteins.

The ARL3 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, displaying distinct ciliary and pericentrosomal staining consistent with its trafficking role. NSJ Bioreagents provides this antibody as a validated reagent for molecular, cellular, and vision research. By enabling precise detection of ADP-ribosylation factor-like protein 3, the ARL3 antibody advances understanding of ciliary transport mechanisms, photoreceptor maintenance, and GTPase-mediated signaling pathways.

UniProt: P36405