DNAJB6 antibody (C-Term)

Catalog No. ABIN7877359

The Rabbit Polyclonal anti-DNAJB6 antibody (ABIN7877359) specifically detects DNAJB6 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DNAJB6 antibody (C-Term) (ABIN7877359)

Target: DNAJB6 (DnaJ (Hsp40) Homolog, Subfamily B, Member 6 (DNAJB6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJB6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DNAJB6 Antibody

Binding Specificity: C-Term

Purpose: DNAJB6 Antibody / DnaJ homolog subfamily B member 6

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DNAJB6 was used as the immunogen for the DNAJB6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DNAJB6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the DNAJB6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for DNAJB6

Target: DNAJB6 (DnaJ (Hsp40) Homolog, Subfamily B, Member 6 (DNAJB6))

Alternative Name: DNAJB6

Background: The DNAJB6 antibody targets DnaJ homolog subfamily B member 6, a molecular chaperone encoded by the DNAJB6 gene. This protein belongs to the Hsp40 (DnaJ) family, which functions as a co-chaperone of Hsp70, facilitating protein folding, quality control, and aggregation prevention. DnaJ homolog subfamily B member 6 plays a key role in maintaining proteostasis and protecting cells from misfolded protein stress. The DNAJB6 antibody enables specific detection of this versatile chaperone, making it an essential reagent for studying protein homeostasis, neurodegeneration, and myopathies.

DnaJ homolog subfamily B member 6 exists in two major isoforms produced by alternative splicing: a long nuclear/cytoplasmic form (DNAJB6a) and a shorter cytoplasmic form (DNAJB6b). Both isoforms cooperate with Hsp70 to stabilize unfolded or aggregation-prone proteins, particularly under stress conditions such as heat shock or oxidative damage. The DNAJB6 antibody detects these isoforms, allowing analysis of expression patterns and subcellular localization in response to proteotoxic stress.

Mutations in DNAJB6 cause limb-girdle muscular dystrophy type D1 (LGMDD1), a progressive neuromuscular disorder characterized by protein aggregation and muscle weakness. The DNAJB6 antibody is crucial for studying the molecular basis of this disease, enabling quantification of mutant and wild-type protein levels and their aggregation propensity. DnaJ homolog subfamily B member 6 has also been shown to suppress polyglutamine aggregation in Huntington's disease models, highlighting its broader neuroprotective role. The DNAJB6 antibody supports investigations into these mechanisms by providing reliable detection in neuronal and muscle tissues.

Beyond its function in proteostasis, DnaJ homolog subfamily B member 6 participates in transcriptional regulation and signal transduction. It interacts with components of the Wnt/beta-catenin pathway, influencing gene expression and cellular differentiation. The DNAJB6 antibody allows exploration of these noncanonical functions, revealing how chaperones integrate protein quality control with developmental signaling. Elevated expression of DNAJB6 has been associated with enhanced stress resistance and reduced metastasis in some cancer cells, further emphasizing its physiological relevance.

The DNAJB6 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It provides clear cytoplasmic and nuclear localization depending on isoform distribution. NSJ Bioreagents supplies this antibody as a high-specificity, reproducible reagent for proteostasis and neuromuscular disease research. By enabling comprehensive study of DnaJ homolog subfamily B member 6, the DNAJB6 antibody supports discovery in protein folding, neurodegeneration, and molecular chaperone biology.

UniProt: O75190