HAND1 antibody (C-Term)

Catalog No. ABIN7877392

The Rabbit Polyclonal anti-HAND1 antibody (ABIN7877392) specifically detects HAND1 in WB. The antibody is reactive with Human samples.

Quick Overview for HAND1 antibody (C-Term) (ABIN7877392)

Target: HAND1 (Heart and Neural Crest Derivatives Expressed 1 (HAND1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAND1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HAND1 Antibody

Binding Specificity: C-Term

Purpose: HAND1 Antibody / Heart and neural crest derivatives-expressed protein 1

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HAND1 was used as the immunogen for the HAND1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HAND1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HAND1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HAND1

Target: HAND1 (Heart and Neural Crest Derivatives Expressed 1 (HAND1))

Alternative Name: HAND1

Background: HAND1 antibody detects Heart and neural crest derivatives-expressed protein 1, a basic helix-loop-helix (bHLH) transcription factor that plays a central role in cardiac morphogenesis, placental development, and cell differentiation. The UniProt recommended name is Heart and neural crest derivatives-expressed protein 1 (HAND1). This transcription factor regulates gene expression programs that control cardiac chamber formation, trophoblast invasion, and embryonic patterning.

Functionally, HAND1 antibody identifies a 215-amino-acid nuclear protein that binds E-box DNA motifs (CANNTG) as a heterodimer with other bHLH transcription factors such as E12 and HAND2. HAND1 activates or represses transcription depending on partner interactions and chromatin context. During embryogenesis, HAND1 is expressed in the developing heart, extraembryonic tissues, and craniofacial mesenchyme, where it directs differentiation of myocardial, trophoblast, and smooth muscle lineages.

The HAND1 gene is located on chromosome 5q33.2 and is transcriptionally regulated by cardiac transcription factors including NKX2-5, GATA4, and MEF2C. HAND1 functions in a dosage-sensitive manner, as either loss or overexpression disrupts normal cardiac looping and ventricular formation. It acts downstream of BMP and WNT signaling pathways that orchestrate early mesoderm and cardiac progenitor specification.

In the heart, HAND1 contributes to left ventricular development and patterning of the outflow tract. It regulates expression of structural and contractile genes required for cardiomyocyte differentiation. In the placenta, HAND1 promotes trophoblast giant cell differentiation and invasion, critical for maternal-fetal exchange. Beyond development, HAND1 has been implicated in cancer biology, where aberrant expression can influence cell proliferation, migration, and angiogenesis through transcriptional reprogramming of developmental pathways.

HAND1 antibody is widely used in developmental biology, cardiology, and transcriptional regulation research. It is suitable for immunohistochemistry, immunofluorescence, and western blotting to detect HAND1 expression in embryonic and differentiated tissues. This antibody supports studies of cardiac gene regulation, lineage specification, and embryonic morphogenesis. It also aids in placental biology research and models of congenital heart defects.

Structurally, HAND1 contains a basic DNA-binding region and a helix-loop-helix dimerization motif that enable sequence-specific DNA binding and partner selection. Post-translational modifications such as phosphorylation influence HAND1 dimerization and transcriptional output. NSJ Bioreagents provides HAND1 antibody reagents validated for use in cardiac development, trophoblast differentiation, and transcriptional network research.

UniProt: O96004

Pathways: Tube Formation