HSPA6 antibody (C-Term)

Catalog No. ABIN7877394

The Rabbit Polyclonal anti-HSPA6 antibody is suitable to detect HSPA6 in samples from Human. It has been validated for WB and FACS.

Quick Overview for HSPA6 antibody (C-Term) (ABIN7877394)

Target: HSPA6 (Heat Shock 70kDa Protein 6 (HSP70B') (HSPA6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPA6 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-HSPA6 Antibody

Binding Specificity: C-Term

Purpose: HSPA6 Antibody / Heat shock 70 kDa protein 6

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA6 was used as the immunogen for the HSPA6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HSPA6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HSPA6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HSPA6

Target: HSPA6 (Heat Shock 70kDa Protein 6 (HSP70B') (HSPA6))

Alternative Name: HSPA6

Background: HSPA6 antibody detects Heat shock 70 kDa protein 6, a stress-inducible molecular chaperone that belongs to the HSP70 family of heat shock proteins. The UniProt recommended name is Heat shock 70 kDa protein 6 (HSPA6). This protein is rapidly upregulated in response to heat shock, oxidative stress, and heavy metal exposure, where it assists in refolding denatured proteins and protecting cells from proteotoxic damage.

Functionally, HSPA6 antibody identifies a 646-amino-acid cytoplasmic and nuclear chaperone that binds unfolded or misfolded polypeptides to prevent aggregation and promote refolding. HSPA6 operates through an ATP-dependent mechanism, alternating between substrate-binding and release cycles coordinated by its nucleotide-binding and substrate-binding domains. Unlike constitutive HSP70 family members, HSPA6 expression is strictly inducible and transient, making it a sensitive marker of acute cellular stress. It cooperates with co-chaperones such as HSP40 and nucleotide exchange factors to restore proteostasis during recovery from stress.

The HSPA6 gene is located on chromosome 1q23.3 and is transcriptionally regulated by heat shock factor 1 (HSF1). Expression is strongly induced by thermal stress and other cytotoxic insults in epithelial, neuronal, and endothelial cells. Under normal physiological conditions, HSPA6 levels are low or undetectable, but during stress, it rapidly accumulates in both the cytoplasm and nucleus, where it stabilizes denatured proteins and facilitates their refolding or degradation.

Pathologically, HSPA6 has been implicated in neuroprotection, cardiac stress response, and cancer cell survival. Upregulation in neurons protects against protein misfolding diseases such as Alzheimer's and Parkinson's, while elevated expression in tumors may contribute to resistance against apoptosis and chemotherapy. In cardiac tissues, HSPA6 supports survival under ischemic or oxidative stress conditions. Research using HSPA6 antibody supports studies in proteostasis, cellular stress pathways, and molecular chaperone biology.

HSPA6 antibody is validated for use in relevant research applications to detect inducible heat shock proteins and examine cellular responses to thermal or oxidative stress. NSJ Bioreagents provides HSPA6 antibody reagents optimized for studies in protein folding, stress signaling, and chaperone-mediated cytoprotection.

UniProt: P17066