HOXB6 antibody (C-Term)

Catalog No. ABIN7877397

The Rabbit Polyclonal anti-HOXB6 antibody is suitable to detect HOXB6 in samples from Mouse, Human and Rat. It has been validated for WB and FACS.

Quick Overview for HOXB6 antibody (C-Term) (ABIN7877397)

Target: HOXB6 (Homeobox B6 (HOXB6))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOXB6 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-HOXB6 Antibody

Binding Specificity: C-Term

Purpose: HOXB6 Antibody / Homeobox protein Hox-B6

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HOXB6 was used as the immunogen for the HOXB6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HOXB6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HOXB6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HOXB6

Target: HOXB6 (Homeobox B6 (HOXB6))

Alternative Name: HOXB6

Background: HOXB6 antibody recognizes Homeobox protein Hox-B6, a sequence-specific transcription factor that directs developmental patterning and cell differentiation. Encoded by the HOXB6 gene on chromosome 17q21.32, this protein belongs to the homeobox (HOX) family of transcription factors that determine positional identity along the anterior-posterior axis during embryogenesis. HOXB6 contains a highly conserved homeodomain that binds specific DNA sequences to regulate expression of genes involved in morphogenesis, hematopoiesis, and organ development. It acts downstream of retinoic acid signaling and cooperates with cofactors such as PBX1 and MEIS1 to control target gene activation.

During development, HOXB6 expression is restricted to specific regions of the embryo corresponding to the thoracic and lumbar domains. It contributes to the specification of vertebrae and rib patterning and influences limb and neural development. In adult tissues, HOXB6 expression is largely downregulated but can be reactivated in certain cell lineages, particularly within the hematopoietic system. Overexpression of HOXB6 has been observed in leukemias, where it drives proliferation and disrupts differentiation by altering transcriptional programs that control cell fate.

The HOXB6 antibody is a valuable reagent for developmental biology and cancer research. Western blot analysis detects a 25 kilodalton band corresponding to HOXB6, and immunohistochemical staining shows nuclear localization consistent with its role as a transcription factor. The antibody supports studies examining HOX gene regulation, retinoic acid signaling, and transcriptional networks controlling tissue differentiation. In experimental systems, HOXB6 expression is used as a marker of posterior HOX cluster activation, which defines segmental identity and developmental timing.

Functionally, HOXB6 binds to enhancer regions of genes that regulate cell cycle progression and differentiation, including MYC and CDKN1A. Dysregulation of HOXB6 is implicated in oncogenesis, particularly in acute myeloid leukemia, where it cooperates with MEIS1 to promote leukemic transformation. In solid tumors, aberrant HOXB6 expression correlates with invasive potential and epithelial-mesenchymal transition. The HOXB6 antibody facilitates detection of these expression changes, enabling characterization of HOX-mediated gene regulation in both normal and malignant cells. NSJ Bioreagents provides this antibody validated for its applications, supporting research into developmental biology and transcriptional control.

UniProt: P17509