MARCKS antibody (C-Term)

Catalog No. ABIN7877435

This Rabbit Polyclonal antibody specifically detects MARCKS in WB, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MARCKS antibody (C-Term) (ABIN7877435)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-MARCKS Antibody

Binding Specificity: C-Term

Purpose: MARCKS Antibody / Myristoylated alanine-rich C-kinase substrate

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MARCKS was used as the immunogen for the MARCKS antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MARCKS antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MARCKS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background: MARCKS antibody detects Myristoylated alanine-rich C-kinase substrate, a key substrate of protein kinase C and an essential regulator of the actin cytoskeleton, cell motility, and signal transduction. MARCKS is a prominent peripheral membrane protein that cycles between membrane-bound and cytosolic states depending on phosphorylation and binding to calcium-calmodulin. The MARCKS antibody is used extensively in studies of cellular signaling, cytoskeletal rearrangement, and neuronal plasticity, providing insight into mechanisms that link phosphorylation signaling to membrane-cytoskeleton interactions.

MARCKS is encoded by the MARCKS gene located on human chromosome 6q22.2. The protein is approximately 32 kilodaltons in size and is characterized by a highly basic effector domain that mediates binding to actin filaments, calmodulin, and acidic phospholipids such as phosphatidylinositol 4,5-bisphosphate (PIP2). N-terminal myristoylation allows MARCKS to anchor reversibly to the plasma membrane. Activation of protein kinase C induces phosphorylation of specific serine residues within the effector domain, causing detachment from the membrane and release of bound PIP2, thus modulating downstream calcium signaling and actin polymerization.

Using the MARCKS antibody, researchers can detect phosphorylated and non-phosphorylated forms of the protein by western blot and immunofluorescence. Western blot typically reveals a single band around 32-35 kilodaltons, while phospho-specific variants of MARCKS migrate slower due to phosphorylation-induced charge differences. Immunostaining shows cortical and membrane localization in unphosphorylated states, transitioning to a diffuse cytoplasmic distribution following stimulation with phorbol esters or calcium ionophores. MARCKS plays critical roles in cell migration, exocytosis, phagocytosis, and neuronal growth cone extension, making it a widely studied effector of signal-dependent cytoskeletal remodeling.

In the nervous system, MARCKS regulates dendritic spine morphology, synaptic vesicle trafficking, and axonal guidance. It is enriched in developing neurons and contributes to long-term potentiation and synaptic plasticity. In non-neuronal tissues, MARCKS is implicated in wound healing, immune cell chemotaxis, and cancer metastasis. Overexpression and dysregulation have been observed in glioblastoma, breast cancer, and inflammatory diseases, highlighting its function as a signaling nexus controlling cell motility and proliferation. NSJ Bioreagents provides a validated MARCKS antibody optimized for western blot, immunofluorescence, and immunohistochemistry, supporting detailed analysis of actin dynamics, phosphorylation signaling, and membrane-cytoskeletal coordination.

UniProt: P29966