Neuronatin (NNAT) (C-Term) antibody

Catalog No. ABIN7877438

This Rabbit Polyclonal antibody specifically detects Neuronatin in IF, IHC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Neuronatin (NNAT) (C-Term) antibody (ABIN7877438)

Target: Neuronatin (NNAT)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details

Binding Specificity: C-Term

Purpose: Neuronatin Antibody / NNAT

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Neuronatin/NNAT was used as the immunogen for the Neuronatin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Neuronatin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Neuronatin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details

Target: Neuronatin (NNAT)

Alternative Name: Neuronatin

Background: Neuronatin antibody detects Neuronatin, a small endoplasmic reticulum-associated protein expressed primarily in the developing nervous system and endocrine tissues. Neuronatin plays an important role in neurogenesis, calcium signaling, and energy metabolism, and is regulated through genomic imprinting with expression derived exclusively from the paternal allele. The Neuronatin antibody is commonly used in neuroscience, developmental biology, and metabolic research to study neuronal differentiation, calcium regulation, and imprinting mechanisms associated with growth and metabolic control.

Neuronatin is encoded by the NNAT gene on human chromosome 20q11.23. Two isoforms, NNAT-alpha and NNAT-beta, arise from alternative splicing and encode proteins of approximately 81 and 70 amino acids, respectively. Both isoforms are hydrophobic, integral membrane proteins localized to the endoplasmic reticulum. Structurally, Neuronatin shares sequence similarity with phospholamban and sarcolipin, suggesting a conserved role in modulating the sarco/endoplasmic reticulum calcium ATPase (SERCA) pump. Through this interaction, Neuronatin influences calcium flux and storage, which are critical for neuronal excitability and hormone secretion.

The Neuronatin antibody reveals protein expression during embryonic and postnatal development in the brain, spinal cord, pituitary gland, and pancreas. Immunostaining shows localization within neuronal soma and processes, as well as in pancreatic beta cells, where Neuronatin contributes to insulin secretion. Western blot analysis typically detects a low molecular weight band corresponding to one or both isoforms. Functional studies demonstrate that Neuronatin enhances intracellular calcium release and supports the differentiation of neural progenitor cells by activating calcium-dependent transcriptional programs.

Beyond its developmental functions, Neuronatin has been implicated in metabolic regulation and energy homeostasis. It modulates endoplasmic reticulum stress responses and may act as a mediator between nutrient sensing and neuronal metabolism. Dysregulation of NNAT expression has been associated with obesity, type 2 diabetes, and neurological disorders such as epilepsy and schizophrenia. Aberrant imprinting or epigenetic silencing of the paternal NNAT allele has been reported in human cancers, indicating a potential role in tumor suppression and differentiation control.

Neuronatin expression persists in adult tissues with high metabolic demand, including hypothalamic nuclei, skeletal muscle, and endocrine pancreas. It functions as a calcium-dependent signal amplifier that adjusts energy utilization and cellular excitability. In neurons, NNAT influences synaptic development and may interact with signaling proteins that control axonal growth. NSJ Bioreagents offers a validated Neuronatin antibody optimized for western blot, immunohistochemistry, and immunofluorescence. This antibody supports comprehensive investigation of calcium-dependent signaling, neural differentiation, and metabolic regulation linked to Neuronatin function.

UniProt: Q16517

Pathways: Positive Regulation of Peptide Hormone Secretion