NUPR1 antibody (C-Term)

Catalog No. ABIN7877439

The Rabbit Polyclonal anti-NUPR1 antibody is suitable to detect NUPR1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for NUPR1 antibody (C-Term) (ABIN7877439)

Target: NUPR1 (Nuclear Protein, Transcriptional Regulator, 1 (NUPR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUPR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-NUPR1 Antibody

Binding Specificity: C-Term

Purpose: NUPR1 Antibody / Nuclear protein 1

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NUPR1 was used as the immunogen for the NUPR1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NUPR1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NUPR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NUPR1

Target: NUPR1 (Nuclear Protein, Transcriptional Regulator, 1 (NUPR1))

Alternative Name: NUPR1

Background: NUPR1 antibody detects Nuclear protein 1, a small chromatin-associated transcriptional regulator induced by cellular stress. Encoded by the NUPR1 gene on chromosome 16p11.2, this intrinsically disordered nuclear protein is activated in response to stress stimuli such as oxidative damage, hypoxia, and nutrient deprivation. NUPR1 modulates gene expression related to cell cycle control, apoptosis, and autophagy, playing a pivotal role in stress adaptation and tumor progression.

Structurally, NUPR1 is a 100-amino-acid protein of approximately 9 kilodaltons lacking defined secondary structure, which enables flexible interactions with DNA and chromatin-modifying enzymes. It acts as a transcriptional cofactor rather than a classical DNA-binding protein, partnering with p300, SMADs, and histone modifiers to influence transcriptional responses. NUPR1 localizes predominantly to the nucleus but can shuttle between compartments depending on stress conditions and post-translational modifications.

The NUPR1 antibody is widely used in oncology, stress biology, and transcription research to study adaptive gene regulation and tumor cell survival. Western blot analysis detects a 9 kilodalton band corresponding to NUPR1, while immunofluorescence demonstrates strong nuclear localization that increases following stress induction. This antibody enables analysis of NUPR1 expression during oxidative and metabolic stress and its contribution to stress-responsive transcription networks.

Functionally, NUPR1 promotes survival under cytotoxic conditions by regulating genes involved in autophagy and anti-apoptotic pathways. It acts downstream of ATF4 and CHOP in the endoplasmic reticulum stress response and influences cell fate decisions by modulating p53-dependent transcription. In cancer, overexpression of NUPR1 supports tumor growth, resistance to chemotherapy, and epithelial-mesenchymal transition (EMT). Conversely, NUPR1 depletion sensitizes tumor cells to oxidative damage and metabolic inhibitors. The NUPR1 antibody provides a key research tool for exploring stress signaling, transcriptional plasticity, and tumor adaptability. NSJ Bioreagents validates this antibody for its applications, ensuring sensitive and reproducible detection across experimental systems.

UniProt: O60356