OPN5 antibody (C-Term)

Catalog No. ABIN7877440

The Rabbit Polyclonal anti-OPN5 antibody (ABIN7877440) specifically detects OPN5 in WB, IF and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for OPN5 antibody (C-Term) (ABIN7877440)

Target: OPN5 (Opsin 5 (OPN5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPN5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-OPN5 Antibody

Binding Specificity: C-Term

Purpose: OPN5 Antibody / Opsin 5 / Neuropsin

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human OPN5 was used as the immunogen for the OPN5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the OPN5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the OPN5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for OPN5

Target: OPN5 (Opsin 5 (OPN5))

Alternative Name: OPN5

Background: OPN5 antibody detects Opsin-5 (also known as Neuropsin or G protein-coupled receptor 136), a light-sensitive G protein-coupled receptor involved in non-visual photoreception. Encoded by the OPN5 gene on chromosome 6p12.3, this protein belongs to the opsin family of photoreceptive GPCRs that use retinaldehyde chromophores to detect light. Opsin-5 absorbs near-ultraviolet light and activates intracellular signaling via inhibitory G proteins (Gi/Go), modulating circadian rhythms and neuroendocrine responses independent of classical retinal photoreceptors.

OPN5 is expressed in brain regions such as the hypothalamus and preoptic area, as well as in peripheral tissues including retina, cornea, skin, and reproductive organs. It functions as a UV-sensitive photoreceptor regulating local circadian oscillators and hormone secretion. In the eye, OPN5 contributes to light entrainment of retinal and non-retinal cells, while in the skin and reproductive tissues, it influences seasonal and environmental light-dependent physiology. The receptor's activation triggers G protein signaling that modulates cyclic AMP levels, gene expression, and ion channel activity.

The OPN5 antibody is a valuable tool for neurobiology, circadian rhythm, and sensory physiology research. Western blot analysis detects a 40 kilodalton band corresponding to Opsin-5, while immunofluorescence and immunohistochemistry reveal membrane localization in neuronal and epithelial tissues. This antibody supports the study of light-dependent signaling outside the visual system and enables mapping of Opsin-5 expression across tissues.

Dysregulation of OPN5 signaling has been linked to altered circadian rhythms, hormonal imbalances, and impaired photic entrainment. In animal models, OPN5 influences melatonin secretion and reproductive seasonality, connecting environmental lighting cues to endocrine function. The OPN5 antibody facilitates the exploration of these mechanisms by providing specific detection of Opsin-5 in experimental systems. NSJ Bioreagents offers this antibody validated for its applications, ensuring consistent and high-quality performance for light-sensing and circadian biology research.

UniProt: Q6U736