PCTP antibody (C-Term)

Catalog No. ABIN7877454

The Rabbit Polyclonal anti-PCTP antibody is suitable to detect PCTP in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for PCTP antibody (C-Term) (ABIN7877454)

Target: PCTP (Phosphatidylcholine Transfer Protein (PCTP))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCTP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCTP Antibody

Binding Specificity: C-Term

Purpose: PCTP Antibody / Phosphatidylcholine transfer protein

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse PCTP was used as the immunogen for the PCTP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PCTP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PCTP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PCTP

Target: PCTP (Phosphatidylcholine Transfer Protein (PCTP))

Alternative Name: PCTP

Background: PCTP antibody detects Phosphatidylcholine transfer protein, a lipid transport protein encoded by the PCTP gene on chromosome 17q11.2. PCTP belongs to the START (steroidogenic acute regulatory protein-related lipid transfer) domain family and is responsible for the intermembrane transfer of phosphatidylcholine, a major phospholipid component of cell membranes. PCTP is highly expressed in liver, macrophages, and skeletal muscle, where it contributes to lipid trafficking, membrane homeostasis, and metabolic regulation. It plays a key role in coordinating lipid transport with energy metabolism, particularly during fasting and lipid oxidation processes.

Structurally, PCTP is a small soluble protein containing a single START domain that forms a hydrophobic cavity for phosphatidylcholine binding. This structure allows PCTP to shuttle phospholipids between intracellular membranes, supporting membrane biogenesis and lipid signaling. It belongs to the phospholipid transfer protein family, which includes STARD2 and STARD10, and interacts with nuclear receptors such as PPAR and LXR that regulate lipid metabolism. Co-localization studies show PCTP distributed in the cytosol and associated with mitochondria and the endoplasmic reticulum.

Functionally, PCTP regulates phospholipid exchange and metabolic signaling. By transferring phosphatidylcholine between membranes, it maintains lipid composition and supports vesicular transport. PCTP also modulates the activity of thioesterase superfamily member THEM2, linking phospholipid transfer to fatty acid oxidation. In macrophages, it influences cholesterol efflux and inflammatory responses, while in hepatocytes, it contributes to VLDL assembly and secretion. PCTP participates in lipid signaling pathways that regulate glucose metabolism, energy storage, and membrane trafficking.

Altered expression of PCTP has been associated with metabolic and cardiovascular diseases. Overexpression promotes lipid accumulation and insulin resistance, while deficiency enhances fatty acid oxidation and improves glucose tolerance. Pathway involvement includes phospholipid transport, lipid metabolism, and mitochondrial energy regulation. Expression of PCTP increases during metabolic stress and fasting, reflecting its role in adaptive lipid handling.

The PCTP antibody from NSJ Bioreagents is a reliable reagent for investigating phospholipid metabolism, intracellular transport, and metabolic regulation.

UniProt: P53808