RAB27B antibody (C-Term)

Catalog No. ABIN7877467

The Rabbit Polyclonal anti-RAB27B antibody (ABIN7877467) specifically detects RAB27B in WB, IHC, FACS, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RAB27B antibody (C-Term) (ABIN7877467)

Target: RAB27B (RAB27B, Member RAS Oncogene Family (RAB27B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB27B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RAB27B Antibody

Binding Specificity: C-Term

Purpose: RAB27B Antibody / Ras-related protein Rab-27B

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAB27B was used as the immunogen for the RAB27B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RAB27B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RAB27B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RAB27B

Target: RAB27B (RAB27B, Member RAS Oncogene Family (RAB27B))

Alternative Name: RAB27B

Background: RAB27B antibody detects Ras-related protein Rab-27B, a member of the Rab family of small GTPases that regulates vesicle docking, secretion, and exocytosis. RAB27B plays key roles in platelet dense granule release, melanosome transport, and hormone secretion. The RAB27B antibody is widely used in cell biology, endocrinology, and neurobiology to study regulated exocytosis and vesicle trafficking.

RAB27B is encoded by the RAB27B gene located on human chromosome 18q21.32. The protein is approximately 221 amino acids long and cycles between active GTP-bound and inactive GDP-bound states. RAB27B localizes to secretory vesicles and interacts with effector proteins such as SYTL2, MLPH, and Slac2 family members, facilitating vesicle tethering to the plasma membrane before exocytosis.

The RAB27B antibody detects a 25 kilodalton protein by western blot and shows vesicular punctate staining under confocal microscopy. RAB27B functions in a wide range of cell types, including platelets, endocrine cells, and neurons, where it regulates secretion of serotonin, insulin, and neurotransmitters. In melanocytes, RAB27B cooperates with RAB27A to control melanosome transport and pigment distribution.

Dysregulation of RAB27B has been linked to cancer metastasis, neurodegenerative disease, and immune dysfunction. Overexpression enhances invasive potential in breast and colorectal cancers by promoting secretion of exosomes that modify the tumor microenvironment. Mutations or reduced function lead to granule secretion defects and pigmentation abnormalities.

Because RAB27B regulates exocytosis and vesicle transport, it serves as a model for understanding intracellular trafficking mechanisms and communication between cells. NSJ Bioreagents provides a validated RAB27B antibody optimized for western blot, immunofluorescence, and vesicle transport assays, supporting studies in secretion, membrane dynamics, and cellular signaling.

UniProt: O00194