WDR55 antibody (C-Term)

Catalog No. ABIN7877499

The Rabbit Polyclonal anti-WDR55 antibody is suitable to detect WDR55 in samples from Human and Mouse. It has been validated for WB and FACS.

Quick Overview for WDR55 antibody (C-Term) (ABIN7877499)

Target: WDR55 (WD Repeat Domain 55 (WDR55))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WDR55 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-WDR55 Antibody

Binding Specificity: C-Term

Purpose: WDR55 Antibody / WD repeat-containing protein 55

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human WDR55 was used as the immunogen for the WDR55 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the WDR55 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the WDR55 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for WDR55

Target: WDR55 (WD Repeat Domain 55 (WDR55))

Alternative Name: WDR55

Background: WDR55 antibody detects WD repeat-containing protein 55, a nucleolar protein essential for ribosome biogenesis and cell proliferation. The UniProt recommended name is WD repeat-containing protein 55 (WDR55). This protein participates in the maturation of 18S ribosomal RNA and assembly of the small ribosomal subunit, contributing to efficient protein synthesis and cell growth.

Functionally, WDR55 antibody identifies a 365-amino-acid protein that localizes to the nucleolus, where it interacts with components of the small subunit (SSU) processome. WDR55's WD-repeat domains form beta-propeller structures that scaffold RNA processing enzymes and ribonucleoprotein complexes necessary for pre-rRNA cleavage and modification.

The WDR55 gene is located on chromosome 2q23.3 and is expressed in actively dividing tissues, including testis, thymus, and embryonic stem cells. By regulating ribosome biogenesis, WDR55 supports developmental processes requiring high translational output and cell proliferation.

Pathologically, loss of WDR55 function disrupts rRNA processing and nucleolar organization, leading to developmental delay, craniofacial abnormalities, and immunodeficiency (as seen in North American Indian childhood cirrhosis-like syndromes). Research using WDR55 antibody supports studies in ribosome assembly, RNA biology, and developmental genetics.

WDR55 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect nucleolar proteins and ribosome assembly factors. NSJ Bioreagents provides WDR55 antibody reagents optimized for studies in RNA metabolism, cell cycle regulation, and nucleolar dynamics.

Structurally, WD repeat-containing protein 55 contains multiple WD repeats forming a propeller-like fold that mediates stable RNA-protein interactions. This antibody enables investigation of WDR55's role in ribosome biogenesis and nucleolar organization.

UniProt: Q9H6Y2