CD73 antibody (full length)

Catalog No. ABIN7877590

The Mouse Monoclonal anti-CD73 antibody (Clone NT5E-2503) (ABIN7877590) specifically detects CD73 in WB, FACS, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CD73 antibody (full length) (ABIN7877590)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: NT5E-2503

Product Details anti-CD73 Antibody

Binding Specificity: full length

Purpose: CD73 Antibody / NT5E (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the CD73 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD73 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background: CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process