EIF2S1 antibody (full length)

Catalog No. ABIN7878204

The Mouse Monoclonal anti-EIF2S1 antibody is suitable to detect EIF2S1 in samples from Human. It has been validated for FACS.

Quick Overview for EIF2S1 antibody (full length) (ABIN7878204)

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: PCRP-EIF2S1-1C11

Product Details anti-EIF2S1 Antibody

Binding Specificity: full length

Purpose: EIF2S1 Antibody / EIF2A

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human EIF2S1/EIF2A protein was used as the immunogen for the EIF2S1 antibody.

Isotype: IgG2a

Application Details

Application Notes: Optimal dilution of the EIF2S1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the EIF2S1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2S1

Background: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2a, eIF2band eIF2g (eukaryotic translation initiation factor 2 a, band g, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2a, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2a controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2a is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C