GCLM antibody (full length)

Catalog No. ABIN7878299

This Mouse Monoclonal antibody specifically detects GCLM in IHC (p). It exhibits reactivity toward Human.

Quick Overview for GCLM antibody (full length) (ABIN7878299)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GCLM-4069

Product Details anti-GCLM Antibody

Binding Specificity: full length

Purpose: GLCLR Antibody / GCLM

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human GLCLR protein was used as the immunogen for the GCLM antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the GLCLR antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the GLCLR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GLCLR

Background: G-glutamylcysteine synthetase (g-GCS) is the rate limiting enzyme for glutathione (L-g-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. g-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human g-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human g-GCS gene encoding the regulatory subunit maps to chromosome 1p22.1. The two subunits of g-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.

UniProt: P48507