Recombinant Involucrin antibody (full length)

Catalog No. ABIN7878462

The Mouse Monoclonal anti-Involucrin antibody is suitable to detect Involucrin in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant Involucrin antibody (full length) (ABIN7878462)

Target: Involucrin (IVL)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Involucrin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: RIVRN-9323

Product Details anti-Involucrin Antibody

Binding Specificity: full length

Purpose: Recombinant IVL Antibody / Involucrin (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: Recombinant human full-length Involucrin protein was used as the immunogen for the recombinant IVL antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the recombinant IVL antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the recombinant IVL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: IVL

Background: It recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

UniProt: P07476