MED7 antibody (full length)

Catalog No. ABIN7878597

The Mouse Monoclonal anti-MED7 antibody is suitable to detect MED7 in samples from Human. It has been validated for ELISA, FACS and IF.

Quick Overview for MED7 antibody (full length) (ABIN7878597)

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED7 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PCRP-MED7-1B8

Product Details anti-MED7 Antibody

Binding Specificity: full length

Purpose: MED7 Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human protein was used as the immunogen for the MED7 antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the MED7 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MED7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MED7

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Alternative Name: MED7

Background: MED7 (Mediator of RNA polymerase II transcription subunit 7), also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, MED7 functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5. Additionally, MED7 regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. MED7 may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases MED7 mRNA-binding affinity. Defects or deletions in the gene encoding MED7 are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of MED7 exist due to alternative splicing events.

UniProt: O43513

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha