Neurogenin 3 antibody (full length)

Catalog No. ABIN7878710

This Mouse Monoclonal antibody specifically detects Neurogenin 3 in ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Neurogenin 3 antibody (full length) (ABIN7878710)

Target: Neurogenin 3 (NEUROG3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neurogenin 3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-NEUROG3-1E10

Product Details anti-Neurogenin 3 Antibody

Binding Specificity: full length

Purpose: Neurogenin 3 Antibody / NGN3

Purification: Protein A/G affinity

Immunogen: Recombinant human full-length protein was used as the immunogen for the Neurogenin 3 antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the Neurogenin 3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Neurogenin 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Neurogenin 3

Target: Neurogenin 3 (NEUROG3)

Alternative Name: Neurogenin 3

Background: This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1. The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

UniProt: Q9Y4Z2