NKX2-1 antibody (full length)

Catalog No. ABIN7878711

The Mouse Monoclonal anti-NKX2-1 antibody is suitable to detect NKX2-1 in samples from Human. It has been validated for IHC (p).

Quick Overview for NKX2-1 antibody (full length) (ABIN7878711)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NKX2-1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM150

Product Details anti-NKX2-1 Antibody

Binding Specificity: full length

Purpose: NKX2.1 Antibody / TTF-1

Purification: Protein G affinity chromatography

Immunogen: Full length rat recombinant protein was used as the immunogen for this NKX2.1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the NKX2.1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the NKX2.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Alternative Name: NKX2.1

Background: Recognizes a protein of 40 kDa, identified as NKX2.1 or Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. Reactivity is also seen in thyroid malignancies.

UniProt: P43699

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour