NPM1 antibody (pThr95) (HRP)

Catalog No. ABIN744407

The Rabbit Polyclonal anti-NPM1 antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect NPM1 in samples from Mouse.

Quick Overview for NPM1 antibody (pThr95) (HRP) (ABIN744407)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPM1 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NPM1 Antibody (HRP)

Binding Specificity: pThr95

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human NPM around the phosphorylation site of Thr95

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM

Background:

Synonyms: Nucleophosmin phospho T95, Nucleophosmin phospho Thr95, p-Nucleophosmin Thr95, B23, B23, MGC104254, NMP1, NMP1, NO38, NPM 1, NPM, NPM_HUMAN, NPM1, Nucleolar Phosphoprotein B23, Nucleolar Phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin nucleolar phosphoprotein B23 numatrin, Nucleophosmin, Nucleophosmin/B23.2, Nucleophosmin/nucleoplasmin family member 1, Nucleoplasmin Family Member 1, Nucleoplasmin Family Member 1, Numatrin, Numatrin, OTTHUMP00000161024, OTTHUMP00000161025, OTTHUMP00000223397, OTTHUMP00000223398, TRK fused gene.

Background: This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009].

Gene ID: 4869

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly