POLE3 antibody (full length)

Catalog No. ABIN7878828

The Mouse Monoclonal anti-POLE3 antibody is suitable to detect POLE3 in samples from Human. It has been validated for IF, FACS and ELISA.

Quick Overview for POLE3 antibody (full length) (ABIN7878828)

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLE3 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), ELISA

Clone: PCRP-POLE3-3D3

Product Details anti-POLE3 Antibody

Binding Specificity: full length

Purpose: CHRAC17 Antibody / POLE3

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human POLE3 protein was used as the immunogen for the CHRAC17 antibody.

Isotype: IgG2a

Application Details

Application Notes: Optimal dilution of the CHRAC17 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CHRAC17 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for POLE3

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Alternative Name: CHRAC17

Background: DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.

UniProt: Q9NRF9

Pathways: DNA Damage Repair