SPIC antibody (full length)

Catalog No. ABIN7879074

The Mouse Monoclonal anti-SPIC antibody is suitable to detect SPIC in samples from Human. It has been validated for IF and FACS.

Quick Overview for SPIC antibody (full length) (ABIN7879074)

Target: SPIC (Spi-C Transcription Factor (Spi-1/PU.1 Related) (SPIC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPIC antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-SPIC-2C5

Product Details anti-SPIC Antibody

Binding Specificity: full length

Purpose: SPIC Antibody / Transcription factor Spi-C

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human Transcription factor Spi-C protein was used as the immunogen for the SPIC antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the SPIC antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SPIC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for SPIC

Target: SPIC (Spi-C Transcription Factor (Spi-1/PU.1 Related) (SPIC))

Alternative Name: SPIC

Background: The Ets transcription factor family is comprised of DNA-binding proteins that influence lymphoid development and activity and bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. Spi-B and Spi-C (also known as SPIC) are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to non-canonical AGAA sites. Spi-C is a 248 amino acid protein that localizes to the nucleus and, like other Ets family members, binds DNA as a monomer and plays a role in transcriptional regulation. Addi- tionally, Spi-C is thought to control the development of red pulp macrophages, thereby contributing to iron homeostasis and red blood cell recycling. Human Spi-C shares 65 % amino acid identity with its mouse counterpart, suggesting a conserved role between species.

UniProt: Q8N5J4