Recombinant Topoisomerase I antibody (full length)

Catalog No. ABIN7879154

This Mouse Monoclonal antibody specifically detects Topoisomerase I in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant Topoisomerase I antibody (full length) (ABIN7879154)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RTOP1MT-488

Product Details anti-Topoisomerase I Antibody

Binding Specificity: full length

Purpose: Recombinant DNA topoisomerase I Antibody / TOP1MT

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human TOP1MT protein was used as the immunogen for the recombinant DNA topoisomerase I antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the recombinant DNA topoisomerase I antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the recombinant DNA topoisomerase I antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: DNA topoisomerase I

Background: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

UniProt: Q969P6

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance