UBE2B antibody (full length)

Catalog No. ABIN7879291

The Mouse Monoclonal anti-UBE2B antibody (Clone PCRP-UBE2B-1C7) (ABIN7879291) specifically detects UBE2B in IF and FACS. The antibody is reactive with Human samples.

Quick Overview for UBE2B antibody (full length) (ABIN7879291)

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBE2B antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: PCRP-UBE2B-1C7

Product Details anti-UBE2B Antibody

Binding Specificity: full length

Purpose: UBE2B Antibody (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human UBE2B protein was used as the immunogen for the UBE2B antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the UBE2B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the UBE2B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for UBE2B

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Alternative Name: UBE2B

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

UniProt: P63146