UBE2B antibody (full length)

Catalog No. ABIN7879292

This Mouse Monoclonal antibody specifically detects UBE2B in IF and FACS. It exhibits reactivity toward Human.

Quick Overview for UBE2B antibody (full length) (ABIN7879292)

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBE2B antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-UBE2B-1C7

Product Details anti-UBE2B Antibody

Binding Specificity: full length

Purpose: UBE2B Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human UBE2B protein was used as the immunogen for the UBE2B antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the UBE2B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the UBE2B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for UBE2B

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Alternative Name: UBE2B

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

UniProt: P63146