WTAP antibody (full length)

Catalog No. ABIN7879326

The Mouse Monoclonal anti-WTAP antibody is suitable to detect WTAP in samples from Human. It has been validated for IHC (p) and FACS.

Quick Overview for WTAP antibody (full length) (ABIN7879326)

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WTAP antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: PCRP-WTAP-1A4

Product Details anti-WTAP Antibody

Binding Specificity: full length

Purpose: WT1-associated protein Antibody / WTAP

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human protein was used as the immunogen for the WT1-associated protein antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the WT1-associated protein antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the WT1-associated protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for WTAP

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Alternative Name: WT1-associated protein

Background: Wilms tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3' UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.

UniProt: Q15007