Recombinant Histone H2A antibody (H2AK118me1)

Catalog No. ABIN7879413

The Rabbit Monoclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ICC.

Quick Overview for Recombinant Histone H2A antibody (H2AK118me1) (ABIN7879413)

Target: Histone H2A

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 31H85

Product Details anti-Histone H2A Antibody

Binding Specificity: H2AK118me1

Purpose: Histone H2A (mono methyl K118) Antibody / HIST1H2AB

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Histone H2A (mono methyl K118) was used as the immunogen for the Histone H2A (mono methyl K118) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Histone H2A (mono methyl K118) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Histone H2A (mono methyl K118) antibody at -20oC.

Target Details for Histone H2A

Target: Histone H2A

Background: Histone H2A (mono methyl K118) antibody detects Histone H2A monomethylated at lysine 118, encoded by the HIST1H2AB gene. Histone H2A is a core nucleosomal protein that forms the nucleosome octamer with histones H2B, H3, and H4. Post translational modifications of histone H2A regulate chromatin structure and gene expression. Monomethylation at lysine 118 represents an important epigenetic mark associated with transcriptional regulation and genome stability. Histone H2A (mono methyl K118) antibody provides researchers with a powerful tool for epigenetic and chromatin research.

Histone modifications such as acetylation, methylation, and phosphorylation function as signals that recruit or repel regulatory proteins. Research using Histone H2A (mono methyl K118) antibody has demonstrated that lysine 118 monomethylation influences transcriptional elongation and DNA damage repair. The modification occurs in the globular domain of histone H2A, suggesting a structural impact on nucleosome stability and interactions with regulatory proteins.

In development, lysine 118 methylation has been linked to regulation of gene expression programs during differentiation. Studies with Histone H2A (mono methyl K118) antibody have shown that abnormal regulation of this mark can disrupt normal transcriptional control, leading to developmental abnormalities. The modification is also dynamically regulated in response to cellular stress, indicating its importance in adaptation and signaling.

Dysregulation of histone H2A methylation, including lysine 118, has been implicated in cancer. Research using Histone H2A (mono methyl K118) antibody has revealed that changes in this modification alter chromatin accessibility and influence oncogene or tumor suppressor gene expression. Because epigenetic states are reversible, histone modifications such as H2A K118 methylation represent potential therapeutic targets in oncology.

Histone H2A (mono methyl K118) antibody is applied in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation maps genome wide distribution of the modification, western blotting distinguishes specific modification states, and immunofluorescence reveals nuclear distribution patterns. These applications make Histone H2A (mono methyl K118) antibody essential for chromatin biology research.

By supplying validated Histone H2A (mono methyl K118) antibody reagents, NSJ Bioreagents supports research into epigenetic regulation, transcription, and cancer. Detection of Histone H2A monomethylated at lysine 118 provides insight into chromatin control mechanisms that underlie development and disease.

UniProt: P04908