Recombinant HIST1H3F antibody (H3K9me2, H3K14ac)

Catalog No. ABIN7879434

The Rabbit Monoclonal anti-HIST1H3F antibody is suitable to detect HIST1H3F in samples from Human. It has been validated for WB.

Quick Overview for Recombinant HIST1H3F antibody (H3K9me2, H3K14ac) (ABIN7879434)

Target: HIST1H3F (Histone Cluster 1, H3f (HIST1H3F))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HIST1H3F antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RM322

Product Details anti-HIST1H3F Antibody

Binding Specificity: H3K9me2, H3K14ac

Purpose: H3K9me2/K14ac Antibody / Dimethyl-Histone H3 (Lys9) + Acetyl-Histone H3 (Lys14)

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys9) and an acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14) was used as the immunogen for the H3K9me2/K14ac antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant H3K9me2/K14ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant H3K9me2/K14ac antibody at -20oC.

Target Details for HIST1H3F

Target: HIST1H3F (Histone Cluster 1, H3f (HIST1H3F))

Alternative Name: Histone H3,Histone H3

Background: This antibody reacts to Histone H3 only when modified by both methylation at lysine 9 and acetylation at lysine 14 (K9me2/K14ac). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), trimethylated Lysine 9 (K9me3), or acetylated Lysine 9/14 only, in histone H3.

UniProt: P84243