PAK4/PAK5/PAK6 (pSer560) antibody

Catalog No. ABIN744473

There is 1 publication for this product available. The Rabbit Polyclonal anti-PAK4/PAK5/PAK6 antibody is suitable to detect PAK4/PAK5/PAK6 in samples from Human, Mouse and Rat. It has been validated for WB, FACS, IF (cc) and IHC (p).

Quick Overview for PAK4/PAK5/PAK6 (pSer560) antibody (ABIN744473)

Target: PAK4/PAK5/PAK6

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: pSer560

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of PAK4 Ser474 [RK(p-S)LV]

Isotype: IgG

Application Details

Application Notes: WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

References

Choi, Yeon, Ryu, Kim, Moon, Lee, Lee, Lee, Heo, Park, Kim: "Repositioning Potential of PAK4 to Osteoclastic Bone Resorption." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, (2015) (PubMed).

Target Details

Target: PAK4/PAK5/PAK6

Sub Type: Cocktail

Background:

Synonyms: PAK4Ser474, PAK5Ser602, PAK6Ser560, p21 activated kinase 4 , p21CDKN1A activated kinase 4, PAK 4, Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs, Serine threonine kinase PAK 4, Serine/threonine protein kinase PAK 4, Serine/threonine protein kinase PAK4, KIAA1142, PAK4_HUMAN.

Background: p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes, including the cell cycle, dynamics of the cytoskeleton, apoptosis, oncogenic transformation, and transcription. All PAK family members are characterized by the presence of p21-binding domain. p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, and lipids, which stimulate autophosphorylation and phosphorylation of exogenous substrates. Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo. Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42. Current data strongly implicates PAK-4 in oncogenesis. PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins.

Gene ID: 10298