Cathepsin F antibody (Middle Region)

Catalog No. ABIN7879509

The Rabbit Polyclonal anti-Cathepsin F antibody is suitable to detect Cathepsin F in samples from Human. It has been validated for WB, IHC and FACS.

Quick Overview for Cathepsin F antibody (Middle Region) (ABIN7879509)

Target: Cathepsin F (CTSF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Cathepsin F Antibody

Binding Specificity: Middle Region

Purpose: CTSF Antibody / Cathepsin F

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cathepsin F/CTSF was used as the immunogen for the CTSF antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CTSF antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CTSF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Cathepsin F

Target: Cathepsin F (CTSF)

Alternative Name: CTSF

Background: CTSF antibody detects Cathepsin F, a lysosomal cysteine protease that mediates protein degradation and turnover in endosomal and lysosomal compartments. The UniProt recommended name is Cathepsin F (CTSF). This protease belongs to the papain-like family of cysteine cathepsins involved in cellular protein recycling and immune antigen processing.

Functionally, CTSF antibody identifies a 484-amino-acid preproenzyme that undergoes proteolytic maturation into active heavy and light chains. Cathepsin F participates in degradation of intracellular and endocytosed proteins, contributing to antigen presentation via MHC class II molecules. It also influences apoptosis, tissue remodeling, and neurodegenerative processes through regulated proteolysis.

The CTSF gene is located on chromosome 11q13.1 and is expressed in brain, liver, spleen, and macrophages. Cathepsin F maintains lysosomal proteolytic balance and participates in innate immune defense through controlled protein breakdown and turnover.

Pathologically, CTSF mutations cause Kufs disease type B, an adult-onset neuronal ceroid lipofuscinosis characterized by progressive neurodegeneration. Dysregulated Cathepsin F expression has also been associated with cancer progression and inflammatory diseases. Research using CTSF antibody supports studies in lysosomal biology, protease regulation, and neurodegenerative mechanisms.

CTSF antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect lysosomal proteases. NSJ Bioreagents provides CTSF antibody reagents optimized for proteolysis, neurobiology, and cellular homeostasis research.

Structurally, Cathepsin F features an N-terminal cystatin-like domain and a papain-like catalytic domain containing the conserved cysteine-histidine-asparagine catalytic triad. This antibody enables examination of CTSF's role in lysosomal degradation and neuronal maintenance.

UniProt: Q9UBX1