CD24 antibody (Middle Region)

Catalog No. ABIN7879510

The Rabbit Polyclonal anti-CD24 antibody (ABIN7879510) specifically detects CD24 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for CD24 antibody (Middle Region) (ABIN7879510)

Target: CD24 (CD24 Molecule (CD24))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD24 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD24 Antibody

Binding Specificity: Middle Region

Purpose: CD24 Antibody

Purification: Antigen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CD24 protein was used as the immunogen for the CD24 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CD24 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CD24 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CD24

Target: CD24 (CD24 Molecule (CD24))

Alternative Name: CD24

Background: CD24 antibody detects Cluster of differentiation 24, a small glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein involved in cell adhesion, immune modulation, and cancer metastasis. Encoded by the CD24 gene on chromosome 6q21, this protein is expressed on developing hematopoietic cells, mature B cells, granulocytes, and various epithelial and neuronal tissues. CD24 mediates cell-cell and cell-matrix interactions by binding to P-selectin and Siglec family receptors, influencing immune cell trafficking and signaling.

Structurally, CD24 is a heavily glycosylated protein lacking a transmembrane domain, anchored to the plasma membrane via a GPI linkage. The carbohydrate moieties of CD24 contribute to its interactions with lectins and adhesion molecules, which regulate lymphocyte homing and activation. In the nervous system, CD24 is involved in axon growth and synaptic plasticity, while in the immune system it acts as a costimulatory molecule modulating T cell responses. CD24 engagement can influence B cell maturation, dendritic cell activation, and apoptosis signaling.

The CD24 antibody is widely used in immunology, oncology, and stem cell biology research to study immune signaling, cancer progression, and differentiation. Flow cytometry using this antibody identifies CD24-positive cell populations, while western blot analysis detects the mature glycoprotein at approximately 35-40 kilodaltons, with size variability due to glycosylation. Immunohistochemistry reveals membrane staining in lymphoid tissues and various carcinomas.

In cancer research, CD24 has emerged as a key marker of tumor-initiating cells and metastasis. Overexpression is associated with aggressive tumor phenotypes in breast, ovarian, and colorectal cancers, where CD24 promotes cell adhesion to P-selectin on platelets and endothelial cells, aiding metastatic spread. Conversely, CD24 expression can modulate immune responses by interacting with Siglec-10, inhibiting innate immune activation. The CD24 antibody is an essential tool for investigating immune regulation, tumor biology, and cell surface glycoprotein signaling. NSJ Bioreagents provides this antibody validated for immunohistochemistry, ensuring high specificity in cellular and molecular studies.

UniProt: P25063

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation