CXXC5 antibody (Middle Region)

Catalog No. ABIN7879512

The Rabbit Polyclonal anti-CXXC5 antibody is suitable to detect CXXC5 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and FACS.

Quick Overview for CXXC5 antibody (Middle Region) (ABIN7879512)

Target: CXXC5 (CXXC Finger Protein 5 (CXXC5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXXC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-CXXC5 Antibody

Binding Specificity: Middle Region

Purpose: CXXC5 Antibody / CXXC-type zinc finger protein 5

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CXXC5 was used as the immunogen for the CXXC5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CXXC5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CXXC5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CXXC5

Target: CXXC5 (CXXC Finger Protein 5 (CXXC5))

Alternative Name: CXXC5

Background: CXXC5 antibody recognizes CXXC-type zinc finger protein 5, a nuclear and cytoplasmic signaling regulator that serves as a negative feedback modulator of the Wnt/beta-catenin pathway. CXXC5 belongs to the CXXC-type zinc finger family, whose members bind unmethylated CpG DNA and influence transcriptional regulation, epigenetic programming, and signal transduction. The CXXC5 antibody is commonly used in developmental biology, cancer, and neurobiology studies to analyze its role in cell differentiation, tissue regeneration, and signal modulation.

CXXC5 is encoded by the CXXC5 gene located on human chromosome 5q31.2. The protein contains a CXXC-type zinc finger motif enabling DNA interaction, and a Dishevelled (DVL)-binding domain that directly inhibits Wnt signaling by preventing DVL-mediated beta-catenin stabilization. This feedback inhibition is essential for maintaining balanced Wnt activity during embryogenesis and adult tissue homeostasis. CXXC5 also acts as a transcriptional co-regulator by associating with DNA methylation machinery, including TET family enzymes, to maintain epigenetic stability.

Using the CXXC5 antibody, researchers can detect a 34-38 kilodalton protein by western blot and identify nuclear and cytoplasmic localization through immunofluorescence microscopy. CXXC5 expression is found in osteoblasts, endothelial cells, neurons, and fibroblasts, where it coordinates growth factor signaling and differentiation. In bone metabolism, CXXC5 inhibits osteoblast differentiation by suppressing Wnt signaling, while in the vascular system it regulates angiogenic responses. Its dysregulation contributes to conditions such as osteoporosis, impaired wound healing, and tumorigenesis.

Functionally, CXXC5 participates in feedback loops linking Wnt, bone morphogenetic protein, and transforming growth factor beta signaling pathways. It also affects neuronal growth cone development and synaptic plasticity. Recent studies suggest that pharmacologic disruption of the CXXC5-DVL interaction can enhance regenerative responses, including hair follicle regeneration and wound repair. NSJ Bioreagents provides a validated CXXC5 antibody optimized for western blot, immunofluorescence, and chromatin immunoprecipitation, supporting comprehensive analysis of Wnt pathway regulation, epigenetic balance, and tissue regeneration mechanisms.

UniProt: Q7LFL8