FASL antibody (Middle Region)

Catalog No. ABIN7879516

The Rabbit Polyclonal anti-FASL antibody (ABIN7879516) specifically detects FASL in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for FASL antibody (Middle Region) (ABIN7879516)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FASL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-FASL Antibody

Binding Specificity: Middle Region

Purpose: FASLG Antibody / FASL / Fas ligand

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FASL/FASLG was used as the immunogen for the FASLG antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the FASLG antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the FASLG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: FASLG

Background: FASLG antibody detects Fas ligand, encoded by the FASLG gene on chromosome 1q23. FASLG antibody is widely applied in apoptosis, immune regulation, and cancer biology. Fas ligand is a type II transmembrane protein belonging to the TNF superfamily that induces apoptosis by binding its receptor Fas (CD95). This ligand-receptor interaction is essential for immune homeostasis, cytotoxic T cell function, and elimination of damaged or malignant cells.

Structurally, Fas ligand is a ~40 kDa protein with a short cytoplasmic tail, a transmembrane region, and a TNF homology domain that mediates trimerization and receptor binding. Fas ligand exists in membrane-bound and soluble forms, the latter generated by proteolytic cleavage. Membrane-bound Fas ligand is the primary inducer of apoptosis, whereas soluble Fas ligand modulates immune signaling and inflammation.

Functionally, Fas ligand is expressed on activated T cells, NK cells, and certain tissues. Engagement of Fas receptor by Fas ligand recruits FADD and initiates caspase-8 activation, leading to apoptosis. Beyond apoptosis, Fas ligand contributes to immune privilege, inflammation, and tissue injury. Researchers use FASLG antibody to study apoptosis, cytotoxic lymphocyte activity, and immune regulation.

Clinically, mutations in FASLG cause autoimmune lymphoproliferative syndrome (ALPS), characterized by defective lymphocyte apoptosis. Dysregulation of Fas ligand contributes to autoimmune disease, transplant rejection, and cancer immune evasion. Tumors exploit Fas ligand expression to induce apoptosis of infiltrating immune cells, aiding immune escape. Because of its dual roles in immune surveillance and pathology, Fas ligand is a target for therapeutic modulation. NSJ Bioreagents supplies FASLG antibody for immunology, apoptosis, and cancer studies.

Experimentally, FASLG antibody is applied in western blotting to detect the ~40 kDa protein, in flow cytometry to measure surface expression, and in immunohistochemistry to study tissue-specific regulation. Functional assays using FASLG antibody evaluate apoptosis induction in cell models.

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity