FUNDC1 antibody (Middle Region)

Catalog No. ABIN7879518

The Rabbit Polyclonal anti-FUNDC1 antibody is suitable to detect FUNDC1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and FACS.

Quick Overview for FUNDC1 antibody (Middle Region) (ABIN7879518)

Target: FUNDC1 (FUN14 Domain Containing 1 (FUNDC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FUNDC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-FUNDC1 Antibody

Binding Specificity: Middle Region

Purpose: FUNDC1 Antibody / FUN14 domain-containing protein 1

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FUNDC1 was used as the immunogen for the FUNDC1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the FUNDC1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the FUNDC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FUNDC1

Target: FUNDC1 (FUN14 Domain Containing 1 (FUNDC1))

Alternative Name: FUNDC1

Background: FUNDC1 antibody detects FUN14 domain-containing protein 1, a mitochondrial outer membrane protein that regulates mitophagy and mitochondrial quality control. Encoded by the FUNDC1 gene on chromosome Xq26.1, this protein serves as a receptor for hypoxia-induced mitophagy, linking damaged or depolarized mitochondria to the autophagic machinery. FUNDC1 interacts with LC3 through its LC3-interacting region (LIR) motif, facilitating selective removal of dysfunctional mitochondria during cellular stress.

Structurally, FUNDC1 contains three transmembrane helices that anchor it to the mitochondrial outer membrane, with its N-terminal domain exposed to the cytosol for binding autophagy-related proteins. Under normoxic conditions, FUNDC1 is phosphorylated at Tyr18 and Ser13, suppressing its interaction with LC3. During hypoxia or mitochondrial depolarization, phosphatases such as PGAM5 dephosphorylate these residues, enhancing its affinity for LC3 and triggering mitophagy. This dynamic post-translational regulation ensures precise control of mitochondrial turnover.

The FUNDC1 antibody is widely used in mitochondrial biology, autophagy, and metabolic research to study mitophagy signaling, organelle homeostasis, and cellular stress adaptation. Western blot analysis detects a 16 kilodalton band corresponding to FUNDC1, while immunofluorescence shows punctate staining along mitochondrial networks. This antibody enables researchers to monitor mitochondrial dynamics and degradation under conditions such as hypoxia, oxidative stress, or metabolic reprogramming.

FUNDC1-mediated mitophagy is essential for maintaining energy balance and preventing accumulation of damaged mitochondria that could release pro-apoptotic or inflammatory signals. Dysregulation of FUNDC1 contributes to diseases such as cardiac ischemia, neurodegeneration, and cancer, where impaired mitophagy affects cell survival and metabolic fitness. The FUNDC1 antibody provides a sensitive reagent for assessing mitophagic flux and mitochondrial remodeling. NSJ Bioreagents validates this antibody for its applications, ensuring reliable detection for studies on mitochondrial quality control and autophagic signaling.

UniProt: Q8IVP5