GSTM2 antibody (Middle Region)

Catalog No. ABIN7879521

The Rabbit Polyclonal anti-GSTM2 antibody is suitable to detect GSTM2 in samples from Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for GSTM2 antibody (Middle Region) (ABIN7879521)

Target: GSTM2 (Glutathione S-Transferase mu 2 (Muscle) (GSTM2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSTM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GSTM2 Antibody

Binding Specificity: Middle Region

Purpose: GSTM2 Antibody / Glutathione S-transferase Mu 2

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse GSTM2 was used as the immunogen for the GSTM2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GSTM2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GSTM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GSTM2

Target: GSTM2 (Glutathione S-Transferase mu 2 (Muscle) (GSTM2))

Alternative Name: GSTM2

Background: GSTM2 antibody detects Glutathione S-transferase Mu 2, a detoxification enzyme that catalyzes the conjugation of glutathione to electrophilic compounds, protecting cells from oxidative damage and xenobiotic stress. Encoded by the GSTM2 gene on chromosome 1p13.3, this enzyme belongs to the mu class of cytosolic glutathione S-transferases (GSTs) and plays a critical role in the metabolism of carcinogens, environmental toxins, and therapeutic drugs. GSTM2 is particularly abundant in liver, kidney, and lung tissues, where it contributes to phase II detoxification.

Structurally, GSTM2 forms homodimers, each monomer containing an N-terminal glutathione-binding site (G-site) and a C-terminal hydrophobic substrate-binding site (H-site). This configuration enables GSTM2 to catalyze the conjugation of reduced glutathione (GSH) to electrophilic substrates, rendering them more water-soluble for excretion. GSTM2 also exhibits peroxidase activity, neutralizing reactive oxygen species and maintaining cellular redox balance.

The GSTM2 antibody is widely used in toxicology, pharmacology, and metabolic research to study oxidative stress responses, detoxification pathways, and enzyme induction mechanisms. Western blot analysis identifies a 26 kilodalton band corresponding to GSTM2, while immunohistochemistry reveals strong cytoplasmic staining in hepatocytes and renal tubular cells. This antibody supports investigations into tissue-specific detoxification and metabolic adaptation to stress and xenobiotics.

Genetic polymorphisms in GSTM2 and related GST genes influence susceptibility to environmental toxins, carcinogens, and drug-induced injury. Reduced expression or functional loss of GSTM2 increases oxidative damage and contributes to disorders such as asthma, cancer, and neurodegenerative disease. Conversely, elevated expression is associated with chemoresistance and altered drug metabolism in tumor cells. The GSTM2 antibody provides a reliable tool for quantifying enzyme expression and understanding its contribution to cellular defense systems. NSJ Bioreagents validates this antibody for its applications, ensuring dependable performance for detoxification and redox biology research.

UniProt: P15626

Pathways: Negative Regulation of Transporter Activity