NUP50 antibody (Middle Region)

Catalog No. ABIN7879533

The Rabbit Polyclonal anti-NUP50 antibody is suitable to detect NUP50 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ICC and IF.

Quick Overview for NUP50 antibody (Middle Region) (ABIN7879533)

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUP50 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NUP50 Antibody

Binding Specificity: Middle Region

Purpose: NUP50 Antibody / Nucleoporin 50

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NUP50 was used as the immunogen for the NUP50 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NUP50 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NUP50 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NUP50

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Alternative Name: NUP50

Background: The NUP50 antibody targets Nuclear pore complex protein Nup50, a nuclear transport factor encoded by the NUP50 gene. Nuclear pore complex protein Nup50 is a dynamic component of the nuclear pore complex (NPC), where it functions in importin-alpha recycling and nuclear import termination. By interacting with importin-beta, RanGTP, and the nucleoporin Nup153, Nup50 promotes the release of import cargo into the nucleoplasm. The NUP50 antibody allows detailed examination of nucleocytoplasmic trafficking, NPC composition, and nuclear transport regulation.

Nuclear pore complex protein Nup50 belongs to the FG (phenylalanine-glycine) repeati 1/2containing family of nucleoporins that mediate selective permeability within the nuclear pore. It localizes to the nuclear basket region of the NPC, where it acts as a cofactor in cargo disassembly and receptor recycling. The NUP50 antibody supports visualization of its distribution along the inner nuclear envelope and nucleoplasmic face, highlighting its mobility during interphase and mitosis. Its high turnover reflects an adaptable role in transport regulation and chromatin organization.

Beyond its role in nuclear transport, Nuclear pore complex protein Nup50 has been implicated in gene regulation. It interacts with chromatin and transcription factors, suggesting a link between nuclear import and transcriptional control. The NUP50 antibody facilitates studies of this regulatory function, allowing researchers to explore how Nup50 coordinates transport with nuclear gene expression. Nup50 depletion alters transcriptional activity and nucleoplasmic organization, leading to cell-cycle arrest and reduced proliferation.

Defects in NUP50 expression have been associated with neurodevelopmental and neuromuscular disorders. Mutations or reduced expression may impair nuclear transport of essential regulatory proteins. The NUP50 antibody supports investigations into these pathologies, enabling assessment of expression and localization in neuronal and muscle tissues. It also serves as a useful marker for nuclear pore integrity in aging and neurodegenerative models.

The NUP50 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing characteristic nuclear-rim and nucleoplasmic staining. NSJ Bioreagents provides this antibody with verified specificity and reproducibility for use in cell biology, molecular genetics, and transport research. By enabling precise detection of Nuclear pore complex protein Nup50, the NUP50 antibody advances understanding of nuclear transport dynamics, pore-complex organization, and nuclear signaling regulation.

UniProt: Q9UKX7

Pathways: Tube Formation