OPN3 antibody (Middle Region)

Catalog No. ABIN7879534

The Rabbit Polyclonal anti-OPN3 antibody (ABIN7879534) specifically detects OPN3 in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for OPN3 antibody (Middle Region) (ABIN7879534)

Target: OPN3 (Opsin 3 (OPN3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-OPN3 Antibody

Binding Specificity: Middle Region

Purpose: OPN3 Antibody / Opsin 3

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human OPN3 was used as the immunogen for the OPN3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the OPN3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the OPN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for OPN3

Target: OPN3 (Opsin 3 (OPN3))

Alternative Name: OPN3

Background: OPN3 antibody detects Opsin-3, a light-sensitive G protein-coupled receptor (GPCR) that belongs to the opsin subfamily of photoreceptors. The UniProt recommended name is Opsin-3 (OPN3). This protein, also known as encephalopsin or panopsin, functions as a non-visual opsin that may mediate light-sensitive signaling in neural and peripheral tissues.

Functionally, OPN3 antibody identifies a 402-amino-acid seven-transmembrane GPCR that binds retinal chromophore and activates intracellular signaling pathways in response to light or metabolic cues. OPN3 couples to heterotrimeric G proteins, influencing cyclic nucleotide levels, calcium signaling, and other downstream responses. Its activity extends beyond traditional photoreception, integrating environmental light signals into physiological regulation of circadian rhythm, metabolism, and hormone secretion.

The OPN3 gene is located on chromosome 1q43 and encodes a protein expressed in brain, skin, testis, and adipose tissue. OPN3 is localized primarily in cell membranes and intracellular vesicles, suggesting context-dependent signaling functions. In the brain, it may modulate neuronal excitability and metabolic responses to light exposure. In skin, OPN3 contributes to phototransduction in melanocytes and keratinocytes, influencing pigmentation and UV response.

Emerging evidence implicates OPN3 in non-visual light sensing that affects circadian entrainment, energy homeostasis, and mood regulation. It may act as a photoreceptor for near-infrared or blue light, mediating cellular adaptation to environmental illumination. In metabolic tissues, OPN3 modulates lipid metabolism and thermogenesis, linking light exposure to systemic energy balance. OPN3 has also been studied for its potential role in reproductive physiology and skin photobiology.

OPN3 antibody is widely used in photoreceptor biology, neuroendocrinology, and GPCR signaling research. It is suitable for immunofluorescence, western blotting, and immunohistochemistry to detect OPN3 localization in neural, skin, and endocrine tissues. This antibody supports research into light-mediated signaling, opsin evolution, and photoregulated physiology. In biomedical studies, OPN3 serves as a molecular link between light sensing and metabolic control.

Structurally, OPN3 exhibits the canonical GPCR topology with seven transmembrane helices, an extracellular retinal-binding lysine, and cytoplasmic domains that interact with G proteins. NSJ Bioreagents provides OPN3 antibody reagents validated for use in light signaling, GPCR function, and circadian regulation research.

UniProt: Q9H1Y3