OSTM1 antibody (Middle Region)

Catalog No. ABIN7879535

This Rabbit Polyclonal antibody specifically detects OSTM1 in WB, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for OSTM1 antibody (Middle Region) (ABIN7879535)

Target: OSTM1 (Osteopetrosis Associated Transmembrane Protein 1 (OSTM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-OSTM1 Antibody

Binding Specificity: Middle Region

Purpose: OSTM1 Antibody / Osteopetrosis-associated transmembrane protein 1

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human OSTM1 was used as the immunogen for the OSTM1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the OSTM1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the OSTM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for OSTM1

Target: OSTM1 (Osteopetrosis Associated Transmembrane Protein 1 (OSTM1))

Alternative Name: OSTM1

Background: OSTM1 antibody detects Osteopetrosis-associated transmembrane protein 1, a lysosomal membrane protein essential for osteoclast function and bone resorption. Encoded by the OSTM1 gene on chromosome 6q21, this protein forms a complex with the chloride channel CLCN7, stabilizing its localization and activity within lysosomal and ruffled-border membranes. The OSTM1-CLCN7 complex is critical for acidification and ion exchange in osteoclasts, processes that drive bone matrix degradation and remodeling. Loss of OSTM1 function results in autosomal recessive osteopetrosis, characterized by dense but brittle bones and defective osteoclast activity.

OSTM1 contains a single transmembrane domain and a large luminal glycoprotein region that protects lysosomal membranes from degradation. It plays a dual role as a chaperone for CLCN7 and as a structural component of lysosomal and endosomal membranes. In addition to skeletal tissues, OSTM1 is expressed in neurons and immune cells, where it contributes to lysosomal biogenesis and degradation of cellular material. Its deficiency leads to impaired lysosomal trafficking, abnormal autophagy, and neurodegeneration.

The OSTM1 antibody is widely used in bone biology, lysosomal function, and neurodegeneration research to study protein trafficking, osteoclast activity, and lysosomal integrity. Western blot analysis identifies a 40-50 kilodalton band corresponding to glycosylated OSTM1, while immunofluorescence reveals punctate lysosomal localization that colocalizes with LAMP1 and CLCN7. This antibody supports detailed studies of lysosomal acidification, bone remodeling, and cellular degradation pathways.

Beyond osteoclast biology, OSTM1 influences autophagic flux and neuronal survival through regulation of lysosomal enzyme maturation and pH balance. The OSTM1 antibody provides an effective tool for exploring the cellular mechanisms underlying osteopetrosis, lysosomal storage disorders, and neurodegenerative diseases. NSJ Bioreagents offers this antibody validated for its applications, ensuring high specificity and reproducibility in lysosomal research.

UniProt: Q86WC4