PABPN1 antibody (Middle Region)

Catalog No. ABIN7879541

This Rabbit Polyclonal antibody specifically detects PABPN1 in WB, IF, IHC, IP, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PABPN1 antibody (Middle Region) (ABIN7879541)

Target: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PABPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PABPN1 Antibody

Binding Specificity: Middle Region

Purpose: PABPN1 Antibody / Polyadenylate-binding protein 2

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PABPN1 was used as the immunogen for the PABPN1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PABPN1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PABPN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PABPN1

Target: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Alternative Name: PABPN1

Background: PABPN1 antibody detects Polyadenylate-binding protein 2, encoded by the PABPN1 gene. Polyadenylate-binding protein 2 is a nuclear protein that binds to the poly(A) tail of RNA and regulates mRNA processing, stability, and translation. PABPN1 antibody provides researchers with a critical reagent for studying RNA metabolism, gene expression, and human disease associated with RNA dysregulation.

Polyadenylate-binding protein 2 is essential for the regulation of poly(A) tail length and stability of nuclear transcripts. Research using PABPN1 antibody has shown that it stimulates poly(A) polymerase activity and ensures that poly(A) tails reach optimal length for nuclear export and translation. By binding RNA, PABPN1 prevents premature deadenylation and degradation, thereby safeguarding mRNA function.

Studies with PABPN1 antibody have revealed that mutations in PABPN1 cause oculopharyngeal muscular dystrophy, a late-onset muscle disorder characterized by progressive weakness of eyelid, pharyngeal, and proximal limb muscles. The disease results from short expansions of a GCN trinucleotide repeat in the gene, which cause protein aggregation and dysfunction. This clinical connection underscores the importance of PABPN1 in human health.

Beyond muscular dystrophy, research using PABPN1 antibody has shown that the protein regulates alternative polyadenylation, influencing transcript diversity and gene expression patterns. Dysregulation of polyadenylation contributes to cancer, neurodegeneration, and developmental disorders, making PABPN1 a key factor in RNA biology.

PABPN1 antibody is widely applied in western blotting, immunohistochemistry, and RNA immunoprecipitation. Western blotting quantifies protein levels in muscle and brain tissue, immunohistochemistry demonstrates nuclear localization, and RNA immunoprecipitation identifies target transcripts. These applications make PABPN1 antibody indispensable for RNA processing research.

By providing validated PABPN1 antibody reagents, NSJ Bioreagents supports studies into polyadenylation, muscular dystrophy, and RNA regulation. Detection of Polyadenylate-binding protein 2 provides researchers with insight into how nuclear RNA-binding proteins regulate transcript stability and disease.

UniProt: Q86U42