SYF2 antibody (Middle Region)

Catalog No. ABIN7879547

This Rabbit Polyclonal antibody specifically detects SYF2 in WB and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for SYF2 antibody (Middle Region) (ABIN7879547)

Target: SYF2 (SYF2 Homolog, RNA Splicing Factor (SYF2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYF2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-SYF2 Antibody

Binding Specificity: Middle Region

Purpose: SYF2 Antibody / Pre-mRNA splicing factor SYF2

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SYF2 was used as the immunogen for the SYF2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SYF2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SYF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SYF2

Target: SYF2 (SYF2 Homolog, RNA Splicing Factor (SYF2))

Alternative Name: SYF2

Background: SYF2 antibody detects Pre-mRNA splicing factor SYF2, a component of the spliceosome complex that participates in mRNA processing, cell cycle progression, and DNA damage response. Encoded by the SYF2 gene on chromosome 1p35.2, this protein acts as a conserved splicing factor that associates with the U5 snRNP complex and other splicing regulators. SYF2 is required for efficient assembly and catalysis of the spliceosome, ensuring accurate intron removal and exon joining during pre-mRNA maturation. Its function extends beyond RNA processing to cell proliferation and genomic stability.

SYF2 interacts with PRPF19, CDC5L, and other components of the spliceosome-associated NTC complex to facilitate transition between spliceosome conformations. It also coordinates splicing with transcription by associating with RNA polymerase II elongation complexes. In addition to its role in RNA processing, SYF2 is involved in G1/S phase progression, likely through regulation of splicing of genes involved in DNA replication and repair. Depletion of SYF2 disrupts normal cell cycle progression, underscoring its role in proliferative control.

The SYF2 antibody is used in RNA biology, cell cycle, and genomics research to study spliceosome assembly and mRNA maturation. Western blot analysis identifies a 24 kilodalton band corresponding to SYF2, and immunofluorescence demonstrates nuclear localization consistent with its splicing function. This antibody enables monitoring of SYF2 expression and localization in studies of transcriptional regulation and RNA processing.

In cancer research, SYF2 has emerged as a proliferation-associated factor, with overexpression reported in breast, lung, and colorectal cancers. It may contribute to tumor progression by promoting efficient splicing of growth-related transcripts. The SYF2 antibody provides a reliable tool for exploring splicing factor regulation, cell cycle control, and their intersections in disease mechanisms. NSJ Bioreagents offers this antibody validated for its applications, ensuring dependable performance across multiple assay types.

UniProt: O95926