APOBEC3C antibody (N-Term)

Catalog No. ABIN7879561

The Rabbit Polyclonal anti-APOBEC3C antibody (ABIN7879561) specifically detects APOBEC3C in WB, IHC, IF, FACS and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for APOBEC3C antibody (N-Term) (ABIN7879561)

Target: APOBEC3C (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3C (APOBEC3C))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOBEC3C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-APOBEC3C Antibody

Binding Specificity: N-Term

Purpose: APOBEC3C Antibody

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human APOBEC3C was used as the immunogen for the APOBEC3C antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the APOBEC3C antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the APOBEC3C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for APOBEC3C

Target: APOBEC3C (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3C (APOBEC3C))

Alternative Name: APOBEC3C

Background: APOBEC3C antibody detects DNA dC-to-dU-editing enzyme APOBEC-3C, a cytidine deaminase involved in antiviral defense, DNA mutagenesis, and innate immune regulation. Encoded by the APOBEC3C gene on chromosome 22q13.1, this enzyme belongs to the APOBEC3 subfamily of cytidine deaminases, which act by deaminating cytidine to uridine in single-stranded DNA. APOBEC3C plays a central role in restricting viral replication, particularly retroviruses and retrotransposons, through cytidine deamination and interference with reverse transcription.

Structurally, APOBEC3C contains a single catalytic zinc-dependent deaminase domain characterized by the H-X-E-X(23-28)-P-C-X2-C motif, which coordinates zinc ions for catalytic activity. It primarily targets cytosines within the 5'-TC context of viral DNA, leading to G-to-A hypermutations that inhibit viral genome integrity. APOBEC3C acts against human immunodeficiency virus type 1 (HIV-1) and other retroelements by editing viral cDNA during replication. Its antiviral activity is antagonized by viral infectivity factor (Vif) proteins that mediate APOBEC degradation via the ubiquitin-proteasome pathway.

The APOBEC3C antibody is used in virology, immunology, and genomic stability research to study intrinsic antiviral immunity and cytidine deamination mechanisms. Western blot analysis detects a 23 kilodalton band corresponding to APOBEC3C, while immunofluorescence shows cytoplasmic and nuclear localization depending on infection or stress conditions. This antibody supports the investigation of APOBEC3C-mediated mutagenesis, viral restriction, and immune regulation.

Beyond antiviral defense, APOBEC3C influences genomic diversity and cancer mutagenesis by contributing to localized DNA editing events. Dysregulation or overexpression of APOBEC family enzymes, including APOBEC3C, has been linked to mutational signatures in various cancers. The APOBEC3C antibody provides a reliable tool for exploring the dual roles of APOBEC3C in immunity and genome evolution. NSJ Bioreagents offers this antibody validated for western blotting, immunohistochemistry, and immunofluorescence, ensuring robust and reproducible detection in studies of antiviral defense and mutation biology.

UniProt: Q9NRW3