DMPK antibody (N-Term)

Catalog No. ABIN7879599

The Rabbit Polyclonal anti-DMPK antibody is suitable to detect DMPK in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for DMPK antibody (N-Term) (ABIN7879599)

Target: DMPK (Dystrophia Myotonica-Protein Kinase (DMPK))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DMPK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DMPK Antibody

Binding Specificity: N-Term

Purpose: DMPK Antibody / Myotonin-protein kinase

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DMPK was used as the immunogen for the DMPK antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DMPK antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the DMPK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for DMPK

Target: DMPK (Dystrophia Myotonica-Protein Kinase (DMPK))

Alternative Name: DMPK

Background: The DMPK antibody targets Myotonin-protein kinase, a serine/threonine kinase encoded by the DMPK gene. This enzyme belongs to the AGC kinase family and plays a central role in muscle structure and function. Myotonin-protein kinase regulates actomyosin organization, calcium homeostasis, and cell signaling in cardiac and skeletal muscle. The DMPK antibody enables detailed exploration of how this kinase influences muscle physiology, signal transduction, and disease mechanisms associated with myotonic dystrophy type 1 (DM1).

Myotonin-protein kinase localizes to the sarcolemma, cytoplasm, and nuclear envelope of muscle cells, where it phosphorylates substrates involved in cytoskeletal organization and ion channel regulation. It contributes to the maintenance of sarcomere integrity and calcium signaling required for normal muscle contraction. The DMPK antibody is used to assess protein expression and localization, providing insights into how loss or mutation of this kinase disrupts muscular homeostasis.

Expansion of CTG trinucleotide repeats in the 3? untranslated region of the DMPK gene causes DM1, a multisystem disorder characterized by muscle weakness, myotonia, and cardiac conduction defects. This repeat expansion leads to toxic RNA accumulation, altered splicing of multiple genes, and reduced DMPK protein levels. The DMPK antibody supports investigations into these molecular effects by allowing direct measurement of protein expression in affected tissues. Studies using this reagent have clarified that decreased Myotonin-protein kinase activity contributes to muscle atrophy and electrical instability in DM1 patients.

Beyond myotonic dystrophy, DMPK signaling intersects with Rho GTPase and MAPK pathways, suggesting roles in cytoskeletal dynamics and stress responses. The DMPK antibody is a valuable reagent for studying these signaling networks and their involvement in cardiac hypertrophy, smooth muscle contractility, and cell migration. In cardiac research, immunohistochemical detection using the DMPK antibody reveals its presence in intercalated discs and perinuclear regions, correlating with its function in maintaining contractile coordination.

Experimental applications for the DMPK antibody include western blotting, immunofluorescence, and immunohistochemistry. These assays permit detailed characterization of Myotonin-protein kinase in tissue and cellular models. NSJ Bioreagents provides the DMPK antibody for consistent, high-specificity detection across research platforms. By enabling accurate quantification and localization of this muscle-specific kinase, the antibody supports ongoing research into muscle physiology, neuromuscular disease, and therapeutic interventions for myotonic dystrophy.

UniProt: P54265

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development