MSI2 antibody (N-Term)

Catalog No. ABIN7879626

The Rabbit Polyclonal anti-MSI2 antibody is suitable to detect MSI2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and FACS.

Quick Overview for MSI2 antibody (N-Term) (ABIN7879626)

Target: MSI2 (Musashi Homolog 2 (MSI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-MSI2 Antibody

Binding Specificity: N-Term

Purpose: MSI2 Antibody / Musashi RNA-binding protein 2

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MSI2 was used as the immunogen for the MSI2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MSI2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MSI2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MSI2

Target: MSI2 (Musashi Homolog 2 (MSI2))

Alternative Name: MSI2

Background: MSI2 antibody detects Musashi RNA-binding protein 2, an evolutionarily conserved RNA-binding protein that regulates mRNA translation and stem cell maintenance. MSI2 belongs to the Musashi family, which controls post-transcriptional gene expression during development and cancer progression. The MSI2 antibody is widely used in stem cell biology, leukemia research, and studies of neural differentiation to examine mRNA regulation and self-renewal mechanisms.

MSI2 is encoded by the MSI2 gene located on human chromosome 17q23.2. The protein is approximately 38 kilodaltons and contains two RNA recognition motifs (RRMs) at the N-terminus that specifically bind to target mRNAs containing UAG motifs in their 3' untranslated regions. Through this interaction, MSI2 represses translation of transcripts involved in differentiation while enhancing those promoting proliferation and stemness. MSI2 expression is particularly high in hematopoietic stem cells and neural progenitors.

The MSI2 antibody detects a 38 kilodalton band by western blot and shows cytoplasmic and perinuclear staining in stem cell populations and tumor cells. MSI2 regulates asymmetric cell division and maintains an undifferentiated state by antagonizing translational repressors such as PABP and interacting with the translational initiation machinery. In hematopoiesis, MSI2 cooperates with HOXA9 and MYC signaling to promote leukemic transformation. Overexpression correlates with poor prognosis in acute myeloid leukemia, breast cancer, and colorectal carcinoma.

In the nervous system, MSI2 modulates neurogenesis by controlling translation of key developmental regulators such as NUMB and REST. Loss of MSI2 leads to premature differentiation and reduced progenitor proliferation. Its function is tightly regulated by phosphorylation and interaction with signaling pathways including Notch and mTOR.

Because of its dual role in maintaining stemness and driving oncogenesis, MSI2 serves as a key marker and therapeutic target in cancer stem cell biology. NSJ Bioreagents provides a validated MSI2 antibody optimized for its applications, supporting detailed research into translational control, stem cell regulation, and tumor initiation.

UniProt: Q96DH6