NPW antibody (N-Term)

Catalog No. ABIN7879633

The Rabbit Polyclonal anti-NPW antibody (ABIN7879633) specifically detects NPW in WB and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for NPW antibody (N-Term) (ABIN7879633)

Target: NPW (Neuropeptide W (NPW))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPW antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-NPW Antibody

Binding Specificity: N-Term

Purpose: NPW Antibody / Neuropeptide W

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NPW was used as the immunogen for the NPW antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NPW antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NPW antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NPW

Target: NPW (Neuropeptide W (NPW))

Alternative Name: NPW

Background: NPW antibody detects Neuropeptide W, a bioactive peptide involved in energy homeostasis, stress response, and neuroendocrine regulation. The UniProt recommended name is Neuropeptide W (NPW). This neuropeptide exerts its physiological effects by binding to G protein-coupled receptors NPBWR1 and NPBWR2, activating downstream intracellular signaling cascades that regulate feeding, hormone secretion, and cardiovascular activity.

Functionally, NPW antibody identifies a 69-amino-acid precursor protein that is processed into two active peptides, NPW-23 and NPW-30, differing by their C-terminal truncation. These peptides are expressed in specific regions of the hypothalamus, brainstem, and peripheral tissues such as adrenal gland and stomach. NPW acts as an endogenous ligand for NPBWR1/2, coupling primarily to Galphai/o proteins and modulating cyclic AMP levels and calcium flux.

The NPW gene is located on chromosome 17p13.1 and encodes a prepropeptide that undergoes proteolytic processing and amidation to generate mature NPW peptides. In the central nervous system, NPW contributes to the regulation of feeding behavior, energy expenditure, and stress hormone release. It acts synergistically with leptin and insulin pathways to balance energy intake and expenditure. NPW also modulates arousal, locomotion, and nociceptive processing through brainstem circuits.

In peripheral tissues, NPW influences cardiovascular tone, adrenal steroidogenesis, and glucose metabolism. Its expression is regulated by nutritional state and stress exposure, reflecting its role in maintaining physiological equilibrium. In metabolic research, NPW is recognized as a peptide integrating hypothalamic signaling with peripheral energy regulation. Dysregulated NPW expression has been associated with obesity, stress-related disorders, and endocrine dysfunction.

NPW antibody is widely used in neuroendocrinology, metabolism, and behavioral neuroscience research. It is suitable for immunohistochemistry, ELISA, and western blotting to detect NPW peptide distribution in brain and peripheral tissues. This antibody supports studies of neuropeptide signaling, hypothalamic regulation, and energy balance. In translational research, NPW serves as a potential biomarker for metabolic and stress-related conditions.

Structurally, NPW peptides adopt flexible conformations stabilized by amidation and hydrophobic residues, allowing receptor binding with high affinity. NSJ Bioreagents provides NPW antibody reagents validated for use in neuropeptide signaling, hypothalamic regulation, and metabolic research.

UniProt: Q8N729

Pathways: Feeding Behaviour