Pcp2 antibody (N-Term)

Catalog No. ABIN7879647

The Rabbit Polyclonal anti-Pcp2 antibody is suitable to detect Pcp2 in samples from Mouse, Human and Rat. It has been validated for WB.

Quick Overview for Pcp2 antibody (N-Term) (ABIN7879647)

Target: Pcp2 (PCP2) (Purkinje Cell Protein 2 (PCP2))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pcp2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Pcp2 Antibody

Binding Specificity: N-Term

Purpose: PCP2 Antibody / Purkinje cell protein 2

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PCP2 was used as the immunogen for the PCP2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PCP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PCP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Pcp2

Target: Pcp2 (PCP2) (Purkinje Cell Protein 2 (PCP2))

Alternative Name: PCP2

Background: PCP2 antibody detects Purkinje cell protein 2, a brain-specific regulator of G protein signaling and neuronal function. Encoded by the PCP2 gene on chromosome 19p13.2, this protein is selectively expressed in cerebellar Purkinje cells and retinal bipolar neurons. PCP2, also known as L7, modulates intracellular signaling by binding to Galpha subunits of heterotrimeric G proteins, influencing synaptic transmission and motor coordination.

PCP2 contains a GoLoco motif that enables high-affinity binding to inactive GDP-bound Galpha subunits, acting as a guanine nucleotide dissociation inhibitor (GDI). Through this interaction, it regulates G protein-coupled receptor (GPCR) signaling pathways that affect neuronal excitability and neurotransmitter release. PCP2 expression increases during cerebellar development, coinciding with synapse formation and maturation of Purkinje cells.

The PCP2 antibody is widely used in neurobiology, developmental, and signaling research to investigate cerebellar function, G protein regulation, and neuronal connectivity. Western blot analysis identifies a 17 kilodalton band corresponding to PCP2, while immunohistochemistry shows intense staining in Purkinje cell somata and dendrites, as well as retinal bipolar neurons. This antibody enables precise visualization of neuronal subtypes and signaling dynamics in the cerebellum and retina.

Altered expression of PCP2 has been associated with ataxia, autism spectrum disorders, and visual processing abnormalities, reflecting its role in synaptic signal modulation. The PCP2 antibody provides a sensitive tool for exploring cerebellar physiology and G protein regulation. NSJ Bioreagents validates this antibody for its applications, ensuring robust detection for neuroscience applications.

UniProt: Q8IVA1