Pyruvate Kinase antibody (N-Term)

Catalog No. ABIN7879648

The Mouse Monoclonal anti-Pyruvate Kinase antibody (Clone 11I4C3) (ABIN7879648) specifically detects Pyruvate Kinase in WB, IF, IHC (p) and FACS. The antibody is reactive with Human samples.

Quick Overview for Pyruvate Kinase antibody (N-Term) (ABIN7879648)

Target: Pyruvate Kinase (PK)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Pyruvate Kinase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 11I4C3

Product Details anti-Pyruvate Kinase Antibody

Binding Specificity: N-Term

Purpose: Pyruvate Kinase Antibody / OIP-3 / PKM2

Sequence: KDPVQEAWAE DVDLRVNFAM NVGKAR

Purification: Antigen affinity purified

Immunogen: N-terminal region amino acids KDPVQEAWAEDVDLRVNFAMNVGKAR from the human protein were used as the immunogen for the Pyruvate Kinase antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the Pyruvate Kinase antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Pyruvate Kinase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Pyruvate Kinase

Target: Pyruvate Kinase (PK)

Alternative Name: Pyruvate Kinase

Background: PKM (Pyruvate Kinase, Muscle), also known as OIP-3, PK3 and PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.

UniProt: P14618